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Application Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Medium power)
Mouse CD314 Monoclonal Antibody | anti-CD314 antibody
MOUSE ANTI HUMAN CD314:Low Endotoxin
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Functional Assay, Immunoprecipitation
Synonyms
CD314; Monoclonal Antibody; MOUSE ANTI HUMAN CD314:Low Endotoxin; anti-CD314 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1D11
Form/Format
Low Endotoxin
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
216
Applicable Applications for anti-CD314 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Functional Assays (FN), Immunoprecipitation (IP)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/200
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/200
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
NKL cells.
Fusion Partners
Fusion Partners
Target Species
Human
Preparation and Storage
Store at -20 degree C only. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Application Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Medium power)
Application Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Medium power)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD314: Alexa Fluor 488)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD314: Alexa Fluor 488)
Application Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . High power)
Application Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . High power)
Application Data
(Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . high power)
Application Data
(Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . high power)
Application Data
(Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Low power)
Application Data
(Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Low power)
Application Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Low power)
Application Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Low power)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD314)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD314)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD314:Low Endotoxin)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD314:Low Endotoxin)
Related Product Information for anti-CD314 antibody
Mouse anti Human CD314 antibody, clone 1D11 recognizes CD314, also known as natural killer receptor G2 (NKG2D) and as killer cell lectin-like receptor subfamily K, member 1 (KLRK1). CD314 is a C-type lectin-like activating receptor which is expressed on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. CD314 forms homodimers that signal through an associated DAP10 adaptor protein. Ligands of CD314 include MICA, MICB and UL16 binding protein (ULBP), which are inducibly expressed. Ligand binding toCD314 results in NK cell activation and potent costimulation of effector T cells. Clone 1D11 is reported to inhibit T cell recognition of MICA (Bauer et al. 1999).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,274 Da
NCBI Official Full Name
NKG2-D type II integral membrane protein
NCBI Official Synonym Full Names
KLRC4-KLRK1 readthrough
NCBI Official Symbol
KLRC4-KLRK1
NCBI Protein Information
NKG2-D type II integral membrane protein; NK cell receptor D; NKG2-D-activating NK receptor; killer cell lectin-like receptor subfamily K member 1
UniProt Protein Name
NKG2-D type II integral membrane protein
UniProt Gene Name
KLRK1
UniProt Synonym Gene Names
D12S2489E; NKG2D
UniProt Entry Name
NKG2D_HUMAN
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Product Notes
The CD314 klrk1 (Catalog #AAA11907) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD314 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Functional Assays (FN), Immunoprecipitation (IP). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/200. Researchers should empirically determine the suitability of the CD314 klrk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD314, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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