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Application Data
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Mouse HLA ABC Monoclonal Antibody | anti-HLA ABC antibody

MOUSE ANTI HUMAN HLA ABC:Low Endotoxin

Applications
Immunohistochemistry, ELISA, Flow Cytometry, Functional Assay, Functional Assay, Immunofluorescence, Immunoprecipitation
Synonyms
HLA ABC, Antibody; MOUSE ANTI HUMAN HLA ABC:Low Endotoxin; anti-HLA ABC antibody
Ordering
 
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For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
W6/32
Form/Format
Low Endotoxin
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Applicable Applications for anti-HLA ABC antibody
Immunohistology Frozen*, ELISA (EIA), Flow cytometry (FC/FACS), Functional Assays (FN), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommends the use of acetone fixation for frozen sections.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Purified human tonsil lymphocyte membranes.
Histology Positive Control Tissue
Target Species
Human
Preparation and Storage
Store at -20 degree C only. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor 647)

Application Data

(Staining of human peripheral blood monocytes with Mouse anti Human HLA ABC:Biotin)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor 488)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:FITC)

Application Data

(Staining of peripheral blood lymphocytes with Mouse anti Human HLA-ABC: Low Endotoxin)

Related Product Information for anti-HLA ABC antibody
Mouse anti Human HLA ABC antibody, clone W6/32 recognizes an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells. Mouse anti Human HLA ABC antibody, clone W6/32 is routinely tested in flow cytometry on human peripheral blood lymphocytes.

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Product Notes

The HLA ABC (Catalog #AAA11910) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HLA ABC can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen*, ELISA (EIA), Flow cytometry (FC/FACS), Functional Assays (FN), Immunofluorescence (IF), Immunoprecipitation (IP). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommends the use of acetone fixation for frozen sections. Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100. Researchers should empirically determine the suitability of the HLA ABC for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HLA ABC, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.