Rat CD11b Monoclonal Antibody | anti-CD11b antibody

RAT ANTI MOUSE CD11b:Biotin

Cat. Num. AAA12187

• Gene Names: Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD11b, Antibody; RAT ANTI MOUSE CD11b:Biotin; anti-CD11b antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: M1/70.15
• Form/Format: Biotin; Purified IgG conjugated to Biotin - liquid
• Concentration: IgG concentration 0.1 mg/ml (varies by lot)
• Sequence Length: 1153

• Applicable Applications for anti-CD11b antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Maximum Dilution: Neat
• Perservative Stabilisers: 0.09% Sodium Azide; 1% Bovine Serum Albumin; Preparation
• Immunogen: T cell enriched splenocytes from B10 mice; Fusion Partners
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Application Data

(Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)

Application Data

(Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)

Application Data

(Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD11b:Alexa Fluor 488)

Application Data

(Staining of total mouse peritoneal exudate cells demonstrating labelling of macrophages with Rat anti Mouse CD11b:RPE)

Application Data

(Published customer image: Immunocytochemical characterisation of cultures of mouse microglia. Cultures of microglia were immunostained with anti-CD11b (a) and anti-GFAP (c). Cultures of astrocytes were immunostained with anti-CD11b (c) and anti-GFAP (d). All cells were also counterstained with DAPI (blue) to identify the cells' nuclei.From: Ferger et al. Journal of Neuroinflammation 2010 7:45)

Application Data

(Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b:RPE)

Application Data

(Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD11b:FITC)

Application Data

(Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG . High power)

Application Data

(Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b: Alexa Fluor 647)

Application Data

(Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG . Medium power)

NCBI and Uniprot Product Information

• NCBI GI #: 124956
• NCBI GeneID: 16409
• Molecular Weight: 115,196 Da
• NCBI Official Full Name: Integrin alpha-M
• NCBI Official Synonym Full Names: integrin alpha M
• NCBI Official Symbol: Itgam
• NCBI Official Synonym Symbols: CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
• NCBI Protein Information: integrin alpha-M; CD11 antigen-like family member B; CD11B (p170); CR-3 alpha chain; Mac-1 alpha; cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component receptor 3 alpha-a; complement receptor type 3; leukocyte adhesion receptor MO1; macrophage antigen alpha
• UniProt Protein Name: Integrin alpha-M
• UniProt Gene Name: Itgam
• UniProt Entry Name: ITAM_MOUSE