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Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)
Rat CD11b Monoclonal Antibody | anti-CD11b antibody
RAT ANTI MOUSE CD11b:Low Endotoxin
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Functional Assay, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
Synonyms
CD11b, Antibody; RAT ANTI MOUSE CD11b:Low Endotoxin; anti-CD11b antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
M1/70.15
Form/Format
Low Endotoxin
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
1153
Applicable Applications for anti-CD11b antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Functional Assays (FN), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*
Application Notes
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100;
Immunohistology - Paraffin: Application Note: PLP fixation is recommended for optimal results, see Whiteland et al. for details
Immunohistology - Paraffin: Application Note: PLP fixation is recommended for optimal results, see Whiteland et al. for details
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
T cell enriched splenocytes from B10 mice
Fusion Partners
Fusion Partners
Target Species
Mouse
Preparation and Storage
Store at -20 degree C only. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)
Application Data
(Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD11b:Alexa Fluor 488)
Application Data
(Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD11b:Alexa Fluor 488)
Application Data
(Staining of total mouse peritoneal exudate cells demonstrating labelling of macrophages with Rat anti Mouse CD11b:RPE)
Application Data
(Staining of total mouse peritoneal exudate cells demonstrating labelling of macrophages with Rat anti Mouse CD11b:RPE)
Application Data
(Published customer image: Immunocytochemical characterisation of cultures of mouse microglia. Cultures of microglia were immunostained with anti-CD11b (a) and anti-GFAP (c). Cultures of astrocytes were immunostained with anti-CD11b (c) and anti-GFAP (d). All cells were also counterstained with DAPI (blue) to identify the cells' nuclei.From: Ferger et al. Journal of Neuroinflammation 2010 7:45)
Application Data
(Published customer image: Immunocytochemical characterisation of cultures of mouse microglia. Cultures of microglia were immunostained with anti-CD11b (a) and anti-GFAP (c). Cultures of astrocytes were immunostained with anti-CD11b (c) and anti-GFAP (d). All cells were also counterstained with DAPI (blue) to identify the cells' nuclei.From: Ferger et al. Journal of Neuroinflammation 2010 7:45)
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b:RPE)
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b:RPE)
Application Data
(Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD11b:FITC)
Application Data
(Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD11b:FITC)
Application Data
(Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG . High power)
Application Data
(Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG . High power)
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b: Alexa Fluor 647)
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b: Alexa Fluor 647)
Application Data
(Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG . Medium power)
Application Data
(Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG . Medium power)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
115,196 Da
NCBI Official Full Name
Integrin alpha-M
NCBI Official Synonym Full Names
integrin alpha M
NCBI Official Symbol
Itgam
NCBI Official Synonym Symbols
CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
NCBI Protein Information
integrin alpha-M; CD11 antigen-like family member B; CD11B (p170); CR-3 alpha chain; Mac-1 alpha; cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component receptor 3 alpha-a; complement receptor type 3; leukocyte adhesion receptor MO1; macrophage antigen alpha
UniProt Protein Name
Integrin alpha-M
UniProt Gene Name
Itgam
UniProt Entry Name
ITAM_MOUSE
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Product Notes
The CD11b itgam (Catalog #AAA12219) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD11b can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Functional Assays (FN), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*. Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100; Immunohistology - Paraffin: Application Note: PLP fixation is recommended for optimal results, see Whiteland et al. for details. Researchers should empirically determine the suitability of the CD11b itgam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD11b, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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