Reactivity
Mouse
Specificity
No cross-reaction with other factors.
Sequence Length
578
Assay Type
Sandwich
Detection Range
100 ug/mL-1.56 ug/mL
Sensitivity
Up to 0.5 ug/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for SA elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse SA monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
49,485 Da
NCBI Official Full Name
SA
NCBI Official Synonym Full Names
acyl-CoA synthetase medium-chain family member 3
NCBI Official Symbol
ACSM3
NCBI Official Synonym Symbols
SA; SAH
NCBI Protein Information
acyl-coenzyme A synthetase ACSM3, mitochondrial; protein SA homolog; butyrate--CoA ligase 3; butyryl-coenzyme A synthetase 3; SA hypertension-associated homolog; middle-chain acyl-CoA synthetase 3; SA (rat hypertension-associated) homolog
UniProt Protein Name
Acyl-coenzyme A synthetase ACSM3, mitochondrial
UniProt Gene Name
ACSM3
UniProt Synonym Gene Names
SAH
UniProt Entry Name
ACSM3_HUMAN