Standard Curve (Sample)
IFN gamma elisa kit
Human IFN gamma HS ELISA Kit
Gene Names
IFNG; IFG; IFI
Reactivity
Human. No cross reactivity with other human cytokines.
Synonyms
IFN gamma; Human IFN gamma HS ELISA Kit; IFN-g HS; IFN gamma elisa kit
Reactivity
Human. No cross reactivity with other human cytokines.
Specificity
Ten specificities were tested with concentrations higher than IFNgamma curve concentrations. No cross reaction was observed for concentrations ranging from 2500 to 78.12 pg/ml for IL-1alpha, IL-2, IL-8, IL-12p40, TNFalpha, CD95 Fas, TRAIL, ICAM-1, Gp130 and GM-CSF.
Sequence Length
166
Samples
Sera, plasmas, buffered solutions or cell culture media
Assay Type
Quantitative Sandwich
Sensitivity
<0.69 pg/ml.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IFN gamma elisa kit
Intended Uses: The Human high sensitivity IFNgamma ELISA Kit is to be used for the in-vitro quantitative determination of interferon gamma (IFNgamma) in human sera, plasmas, buffered solutions or cell culture media. The assay will recognize both natural and recombinant human IFNgamma. This kit has been configured for research use only.
Principle of the Assay: The IFNgamma Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for IFNgamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFNgamma concentrations and unknowns are pipetted into these wells. During the first incubation, the IFNgamma antigen and a biotinylated monoclonal antibody specific for IFNgamma are successively incubated. After washing, the enzyme (streptavidin-peroxydase) is added. All the unbound enzyme is removed by washing and the first amplification step is performed by adding the Biotine-Tyramine reagent. Under the action of HRP, a biotine polymerisation reaction occurs in the region of the HRP linked to the detection antibody. After washing the second amplification step is performed and the polymerised biotine is revealed by a new streptavidin-HRP step. Finally after washing, the substrate is added. The intensity of this coloured product is directly proportional to the concentration of IFNgamma present in the samples.
Principle of the Assay: The IFNgamma Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for IFNgamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFNgamma concentrations and unknowns are pipetted into these wells. During the first incubation, the IFNgamma antigen and a biotinylated monoclonal antibody specific for IFNgamma are successively incubated. After washing, the enzyme (streptavidin-peroxydase) is added. All the unbound enzyme is removed by washing and the first amplification step is performed by adding the Biotine-Tyramine reagent. Under the action of HRP, a biotine polymerisation reaction occurs in the region of the HRP linked to the detection antibody. After washing the second amplification step is performed and the polymerised biotine is revealed by a new streptavidin-HRP step. Finally after washing, the substrate is added. The intensity of this coloured product is directly proportional to the concentration of IFNgamma present in the samples.
Product Categories/Family for IFN gamma elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,348 Da
NCBI Official Full Name
interferon gamma
NCBI Official Synonym Full Names
interferon, gamma
NCBI Official Symbol
IFNG
NCBI Official Synonym Symbols
IFG; IFI
NCBI Protein Information
interferon gamma
UniProt Protein Name
Interferon gamma
UniProt Gene Name
IFNG
UniProt Synonym Gene Names
IFN-gamma
UniProt Entry Name
IFNG_HUMAN
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Product Notes
The Human. No cross reactivity with other human cytokines. IFN gamma ifng (Catalog #AAA13472) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA13472 ELISA Kit recognizes Human. No cross reactivity with other human cytokines. IFN gamma. It is sometimes possible for the material contained within the vial of "IFN gamma, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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