Mouse PGP9.5 / UchL1 Monoclonal Antibody | anti-UCHL1 antibody

PGP9.5 / UchL1 (pan-Neuronal Marker) Mouse Monoclonal Antibody

Cat. Num. AAA13809

• Gene Names: UCHL1; NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
• Reactivity: Human, Mouse, Rat, Cow, Pig, Dog
• Applications: Western Blot, Immunohistochemistry
• Synonyms: PGP9.5 / UchL1; Monoclonal Antibody; PGP9.5 / UchL1 (pan-Neuronal Marker) Mouse Monoclonal Antibody; Gracile Axonal Dystrophy; Neuron Cytoplasmic Protein 9.5; Park5; Parkinson Disease 5; PGP95; Protein Gene Product 9.5; Ubiquitin Carboxyl-terminal Esterase L1; Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1; Ubiquitin Thioesterase L1; Ubiquitin Thiolesterase L1; anti-UCHL1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Cow, Pig, Dog
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 31A3
• Specificity: This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.
• Form/Format: 1.0mg/mL of Ab purified from Bioreactor Concentrate by Protein A/G.
• Sequence Length: 223

• Applicable Applications for anti-UCHL1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Formalin
• Application Notes: Western Blot (0.5-1ug/ml); Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Cytoplasmic. Endoplasmic Reticulum membrane
• Immunogen: Native UchL1 (PGP9.5) protein from brain
• Hu-Chromosome Location: 4p13
• Positive Control: T98G Cells Cerebellum
• Preparation and Storage: Antibody without azide - store at -20 to -80 degree C. Non-hazardous. No MSDS required.

IF (Immunofluorescence)

(Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red))

FCM (Flow Cytometry)

(Flow Cytometric Analysis of T98G cells using Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

WB (Western Blot)

(Western Blot Analysis of human brain tissue lysate using Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3).)

WB (Western Blot)

(Western Blot of UchL1 (PGP9.5) in 1) human, 2) mouse and 3) rat brain Lysate using UchL1 Monoclonal Antibody (31A3).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Cerebellum stained with Pgp9.5 / UchL1 Monoclonal Antibody (31A3).)

References

Day IN et. al. Biochem Society Trans 14:350-351 (1986

NCBI and Uniprot Product Information

• NCBI GI #: 21361091
• NCBI GeneID: 7345
• NCBI Accession #: NP_004172.2
• NCBI GenBank Nucleotide #: NM_004181.4
• UniProt Accession #: P09936; Q4W5K6; Q71UM0
• Molecular Weight: 20-30kDa
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L1
• NCBI Official Synonym Full Names: ubiquitin C-terminal hydrolase L1
• NCBI Official Symbol: UCHL1
• NCBI Official Synonym Symbols: NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L1
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L1
• UniProt Gene Name: UCHL1
• UniProt Synonym Gene Names: UCH-L1; PGP9.5
• UniProt Entry Name: UCHL1_HUMAN