Chicken Ubiquitin C-terminal Hydrolase 1 (UCHL1) ck Polyclonal Antibody | anti-UCHL1 antibody

Anti-Ubiquitin C Terminal Hydrolase 1 (UCHL1)

Cat. Num. AAA14227

• Gene Names: UCHL1; NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5
• Reactivity: Bovine, Human, Mouse, Pig, Rat; Expected Reactivity: Canine, Feline, Goat, Guinea Pig, Hamster, Horse, Non-Human Primate, Rabbit, Sheep, Vole
• Applications: Western Blot, Immunofluorescence
• Purity: Total IgY fraction
• Synonyms: Ubiquitin C-terminal Hydrolase 1 (UCHL1) ck; Polyclonal Antibody; Anti-Ubiquitin C Terminal Hydrolase 1 (UCHL1); anti-UCHL1 antibody

• Host: Chicken
• Reactivity: Bovine, Human, Mouse, Pig, Rat; Expected Reactivity: Canine, Feline, Goat, Guinea Pig, Hamster, Horse, Non-Human Primate, Rabbit, Sheep, Vole
• Clonality: Polyclonal
• Specificity: Specific for the ~24kDa UCHL1 protein in Western blots in bovine, human, mouse, rat. It is expected that it will work on other mammalian tissues.
• Purity/Purification: Total IgY fraction
• Form/Format: Liquid. 100ul antibody per vial PBS + 10 mM NaN₃. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 223

• Applicable Applications for anti-UCHL1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:2,500; IF: 1:500
• Immunogen: Recombinant full length human UCHL1 purified from E. coli.
• Preparation and Storage: Store at -20C; Stable for at least one year.

IF (Immunofluorescence)

(Immunostaining of mixed neuron/glial cultures stained with anti-UCHL1 antibody (AAA14227), green, 1:500) and rabbit anti-GFAP antibody , red, 1:1000. The blue stains nuclear DNA. The anti-UCHL1 stains strongly the cell body and dendrites of neurons, while anti-GFAP specifically labels astrocytes.)

WB (Western Blot)

(Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~24 kDa UCHL1 protein.)

Related Product Information for anti-UCHL1 antibody

Target Description: Ubiquitin C-terminal hydrolase 1 (UCHL1) is also known as ubiquitin carboxyl esterase L1, ubiquitin thiolesterase, neuron-specific protein PGP9.5 and Park5. It was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5 (1). It was later found that ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein (2). The ubiquitin C-terminal hydrolases cleave ubiquitin from other molecules. Regulation of the ubiquitin pathway is very important and many disease states are associated with defects in this pathway. Genetic knockout of UCHL1 in mice results in a motor neuron degeneration similar to the spontaneous gracile axonal dystrophy (gad) mutant mice (3). Point mutations in the UCHL1 gene are associated with some forms of human Parkinson's disease (4). Since UCHL1 is heavily expressed in neurons, it is released in large amounts following injury or degeneration, so the detection of UCHL1 in CSF and other bodily fluids can be used as a biomarker.

References

• Doran JF, Jackson P, Kynoch PA, Thompson RJ. Isolation of PGP 9.5, a new human neurone-specific protein detected by high-resolution two-dimensional electrophoresis. J Neurochem. 40:1542-7 (1983).
• Wilkinson KD, Lee KM, Deshpande S, Duerksen-Hughes P, Boss JM, Pohl J. The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase. Science. 1989 246:670-3 (1989).
• Kurihara LJ, Kikuchi T, Wada K, Tilghman SM. Loss of Uch-L1 and Uch-L3 leads to neurodegeneration, posterior paralysis and dysphagia. Hum Mol Genet. 10:1963-70 (2001).
• Liu Y, Fallon L, Lashuel HA, Liu Z, Lansbury PT Jr. The UCH-L1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility. Cell 111:209-18 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 21361091
• NCBI GeneID: 7345
• NCBI Accession #: NP_004172.2
• NCBI GenBank Nucleotide #: NM_004181.4
• UniProt Accession #: P09936; Q4W5K6; Q71UM0
• Molecular Weight: 24kDa
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L1
• NCBI Official Synonym Full Names: ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• NCBI Official Symbol: UCHL1
• NCBI Official Synonym Symbols: NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin thioesterase L1; epididymis luminal protein 117; neuron cytoplasmic protein 9.5; ubiquitin C-terminal hydrolase
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L1
• UniProt Gene Name: UCHL1
• UniProt Synonym Gene Names: UCH-L1; PGP9.5
• UniProt Entry Name: UCHL1_HUMAN