Standard Curve (Sample)
Human Collagen Type VII (COL7) ELISA Kit | COL7 elisa kit
Human Collagen Type VII (COL7) ELISA Kit
Gene Names
COL7A1; EBD1; EBR1; EBDCT; NDNC8
Reactivity
Human
Synonyms
Collagen Type VII (COL7); Human Collagen Type VII (COL7) ELISA Kit; COL7-A1; COL7A1; EBD1; EBDCT; EBR1; Long-chain collagen; Epidermolysis Bullosa; Dystrophic; Dominant And Recessive; Collagen Alpha-1(VII)chain; COL7 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of COL7. No significant cross-reactivity or interference between COL7 and analogues was observed.
Sequence Length
2944
Samples
Serum, plasma, tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
< 0.069ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level COL7 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level COL7 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for COL7 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of COL7 in human serum, plasma, tissue homogenates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to COL7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to COL7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of COL7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to COL7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to COL7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of COL7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
292,267 Da
NCBI Official Full Name
collagen alpha-1(VII) chain
NCBI Official Synonym Full Names
collagen type VII alpha 1 chain
NCBI Official Symbol
COL7A1
NCBI Official Synonym Symbols
EBD1; EBR1; EBDCT; NDNC8
NCBI Protein Information
collagen alpha-1(VII) chain
UniProt Protein Name
Collagen alpha-1(VII) chain
UniProt Gene Name
COL7A1
UniProt Synonym Gene Names
LC collagen
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Product Notes
The Human COL7 col7a1 (Catalog #AAA13974) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA13974 ELISA Kit recognizes Human COL7. It is sometimes possible for the material contained within the vial of "Collagen Type VII (COL7), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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