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IHC (Immunohistochemistry)
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IHC (Immunohistochemistry) (Immunohistochemical staining of human thyroid cancer using anti-CD52 antibody AAA14187 Formalin fixed human thyroid cancer slices were were stained with a AAA14187-10.0 at 5 ug/ml.)

Rat CD52 Antibody | anti-CD52 antibody

Anti-CD52 [Campath-1H], Rat IgG2b

Gene Names
CD52; CDW52; EDDM5
Reactivity
Human, Rhesus Monkey, Cynomolgus Monkey
Applications
ELISA, Immunohistochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
Purity
Protein A affinity purified
Synonyms
CD52; Anti-CD52 [Campath-1H]; Rat IgG2b; CDW52; Cambridge pathology 1 antigen; Epididymal secretory protein E5; Human epididymis-specific protein 5; YTH66.9HL; Alemtuzumab; anti-CD52 antibody
Ordering
For Research Use Only!
Host
Rat
Reactivity
Human, Rhesus Monkey, Cynomolgus Monkey
Isotype
Rat IgG2b, Kappa
Clone Number
Campath-1H
Specificity
Human CD52 antigen, also known as CAMPATH-1. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD52 antibody
Cytotoxicity (CT), ELISA (EIA), Immunohistochemistry-Frozen (IHC-F), Immunohistochemistry-Paraffin (IHC-P), in vivo, Flow Cytometry (FC/FACS), Western Blot (WB)
Immunogen
Human lymphocytes.
Origin Pub PMID
10366506
Chimeric Use Statement
This is a research-grade biosimilar. This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

IHC (Immunohistochemistry)

(Immunohistochemical staining of human thyroid cancer using anti-CD52 antibody AAA14187 Formalin fixed human thyroid cancer slices were were stained with a AAA14187-10.0 at 5 ug/ml.)

IHC (Immunohistochemistry) (Immunohistochemical staining of human thyroid cancer using anti-CD52 antibody AAA14187 Formalin fixed human thyroid cancer slices were were stained with a AAA14187-10.0 at 5 ug/ml.)

IF (Immunofluorescence)

(Immunofluorescent staining of rat thymus using anti-CD52 antibody AAA14187 Formaldehyde-fixed rat thymus slices were stained with AAA14187-10.0 at 5 ug/ml and detected with a FITC-conjugated secondary antibody. AAA14187 shows cross-reactivity with rat.)

IF (Immunofluorescence) (Immunofluorescent staining of rat thymus using anti-CD52 antibody AAA14187 Formaldehyde-fixed rat thymus slices were stained with AAA14187-10.0 at 5 ug/ml and detected with a FITC-conjugated secondary antibody. AAA14187 shows cross-reactivity with rat.)

FCM (Flow Cytometry)

(Flow-cytometry using anti-CD52 antibody Campath-1H (AAA14187) Cynomolgus monkey lymphocytes were stained with an isotype control or the rabbit-chimeric version of Campath-1H at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.)

FCM (Flow Cytometry) (Flow-cytometry using anti-CD52 antibody Campath-1H (AAA14187) Cynomolgus monkey lymphocytes were stained with an isotype control or the rabbit-chimeric version of Campath-1H at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.)

FCM (Flow Cytometry)

(Flow-cytometry using anti-CD52 antibody Campath-1H Rhesus monkey lymphocytes were stained with an isotype control or the rabbit-chimeric version of Campath-1H at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.)

FCM (Flow Cytometry) (Flow-cytometry using anti-CD52 antibody Campath-1H Rhesus monkey lymphocytes were stained with an isotype control or the rabbit-chimeric version of Campath-1H at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.)

IHC (Immunohistochemistry)

(Immunohistochemical staining of rat thymus tissue using anti-CD52 antibody (AAA14187) Campath-1H. Anti-CD52 staining of formaldehyde fixed paraffin embedded rat thymus tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (AAA14187-10.0) was used to stain samples at a concentration of 5 ug/ml.)

IHC (Immunohistochemistry) (Immunohistochemical staining of rat thymus tissue using anti-CD52 antibody (AAA14187) Campath-1H. Anti-CD52 staining of formaldehyde fixed paraffin embedded rat thymus tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (AAA14187-10.0) was used to stain samples at a concentration of 5 ug/ml.)

IHC (Immunohistochemistry)

(Immunohistochemical staining of rat spleen tissue using anti-CD52 antibody (AAA14187) Campath-1H. Anti-CD52 staining of formaldehyde fixed paraffin embedded rat spleen tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (AAA14187-10.0) was used to stain samples at a concentration of 5 ug/ml.)

IHC (Immunohistochemistry) (Immunohistochemical staining of rat spleen tissue using anti-CD52 antibody (AAA14187) Campath-1H. Anti-CD52 staining of formaldehyde fixed paraffin embedded rat spleen tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (AAA14187-10.0) was used to stain samples at a concentration of 5 ug/ml.)

IHC (Immunohistochemistry)

(Immunohistochemical staining of rat epididymis tissue using anti-CD52 antibody (AAA14187) Campath-1H. Anti-CD52 staining of formaldehyde fixed paraffin embedded rat epididymis tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (AAA14187-10.0) was used to stain samples at a concentration of 5 ug/ml.)

IHC (Immunohistochemistry) (Immunohistochemical staining of rat epididymis tissue using anti-CD52 antibody (AAA14187) Campath-1H. Anti-CD52 staining of formaldehyde fixed paraffin embedded rat epididymis tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (AAA14187-10.0) was used to stain samples at a concentration of 5 ug/ml.)

IF (Immunofluorescence)

(Immunofluorescence staining of fixed rat thymus tissue with anti-CD52 antibody Campath-1H (AAA14187) Immunofluorescence analysis of fixed rat thymus tissue stained with the chimeric human IgG1 version of Campath-1H (AAA14187) at 5 ug/ml followed by a FITC conjugated secondary antibody, showing membrane and cytoplasmic staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right; AAA14187 (FITC), DAPI and merged channels.)

IF (Immunofluorescence) (Immunofluorescence staining of fixed rat thymus tissue with anti-CD52 antibody Campath-1H (AAA14187) Immunofluorescence analysis of fixed rat thymus tissue stained with the chimeric human IgG1 version of Campath-1H (AAA14187) at 5 ug/ml followed by a FITC conjugated secondary antibody, showing membrane and cytoplasmic staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right; AAA14187 (FITC), DAPI and merged channels.)
Product Categories/Family for anti-CD52 antibody
References
Riechmann L, Clark M, Waldmann H, Winter G. Reshaping human antibodies for therapy. Nature. 1988 Mar 24;332(6162):323-7. PMID:3127726

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
6,614 Da
NCBI Official Full Name
CAMPATH-1 antigen
NCBI Official Synonym Full Names
CD52 molecule
NCBI Official Symbol
CD52
NCBI Official Synonym Symbols
CDW52; EDDM5
NCBI Protein Information
CAMPATH-1 antigen
UniProt Protein Name
CAMPATH-1 antigen
UniProt Gene Name
CD52
UniProt Synonym Gene Names
CDW52; HE5; He5

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Product Notes

The CD52 cd52 (Catalog #AAA14187) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD52 [Campath-1H], Rat IgG2b reacts with Human, Rhesus Monkey, Cynomolgus Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's CD52 can be used in a range of immunoassay formats including, but not limited to, Cytotoxicity (CT), ELISA (EIA), Immunohistochemistry-Frozen (IHC-F), Immunohistochemistry-Paraffin (IHC-P), in vivo, Flow Cytometry (FC/FACS), Western Blot (WB). Researchers should empirically determine the suitability of the CD52 cd52 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD52, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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