Application Data
Chicken Microtubule Associated Protein 2 (MAP2) Polyclonal Antibody | anti-MAP2 antibody
Anti-Microtubule Associated Protein 2 (MAP2)
Gene Names
MAP2; MAP2A; MAP2B; MAP2C
Reactivity
Bovine, Human, Mouse, Rat.
Applications
Western Blot, Immunofluorescence
Synonyms
Microtubule Associated Protein 2 (MAP2); Polyclonal Antibody; Anti-Microtubule Associated Protein 2 (MAP2); anti-MAP2 antibody
Host
Chicken
Reactivity
Bovine, Human, Mouse, Rat.
Clonality
Polyclonal
Specificity
Specific for the ~ 280k MAP2 protein in Western Blots of rat brain homogenate.
Form/Format
PBS+ 10 mM NaN3
Total IgY fraction
Total IgY fraction
Applicable Applications for anti-MAP2 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
Quality Control Test:
Western blots performed on each lot.
Working Dilutions:
WB: 1:20,000
IF: 1:2,500
Western blots performed on each lot.
Working Dilutions:
WB: 1:20,000
IF: 1:2,500
Packaging
100ul antibody per vial +10 mM NaN3. Adequate amount of material to conduct 10-mini Western Blots.
Immunogen
Recombinant bovine MAP2 protein expressed in and purified from E.coli.
Preparation and Storage
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20 degree or -80 degree C, while keeping a working aliquot stored at 4 degree C for short term. Avoid freeze/thaw cycles.
Application Data
Application Data
Related Product Information for anti-MAP2 antibody
Chicken polyclonal antibody
Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein ? (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein ? (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine- Lenore V. et al., 2006).
Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein ? (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein ? (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine- Lenore V. et al., 2006).
References
• Kindler S, Gardner CC (1994) Four repeat MAP2 isoforms in human and rat brain. Brain Res Mol Brain Res. 26(1-2):218-224.
• Fontaine-Lenore V, Chambraud B, Fellous A, David S, Duchossoy Y, Baulieu EE, Robel P (2006) Microtubule-associated protein 2 (MAP2) is a neurosteroid. Proc Natl Acad Sci USA 103(12):4711-6.
• Fontaine-Lenore V, Chambraud B, Fellous A, David S, Duchossoy Y, Baulieu EE, Robel P (2006) Microtubule-associated protein 2 (MAP2) is a neurosteroid. Proc Natl Acad Sci USA 103(12):4711-6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
280 kDa
NCBI Official Full Name
microtubule-associated protein 2 isoform 5
NCBI Official Synonym Full Names
microtubule-associated protein 2
NCBI Official Symbol
MAP2
NCBI Official Synonym Symbols
MAP2A; MAP2B; MAP2C
NCBI Protein Information
microtubule-associated protein 2; MAP-2
UniProt Protein Name
Microtubule-associated protein 2
UniProt Gene Name
MAP2
UniProt Synonym Gene Names
MAP-2
UniProt Entry Name
MTAP2_HUMAN
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Product Notes
The MAP2 map2 (Catalog #AAA14235) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The Anti-Microtubule Associated Protein 2 (MAP2) reacts with Bovine, Human, Mouse, Rat. and may cross-react with other species as described in the data sheet. AAA Biotech's Microtubule Associated Protein 2 (MAP2) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Quality Control Test: Western blots performed on each lot. Working Dilutions: WB: 1:20,000 IF: 1:2,500. Researchers should empirically determine the suitability of the MAP2 map2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Microtubule Associated Protein 2 (MAP2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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