Application Data
(S100A8 in NIH-3T3 mouse cell line: S100A8 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using 10ug/mL AAA14670 for 3 hours at RT. Cells were stained (red) and counterstained with DAPI (blue).)
Rat anti-Mouse S100A8 Monoclonal Antibody | anti-S100A8 antibody
S100A8 (MRP-8)
Gene Names
S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG; S100A8
Reactivity
Mouse
Applications
ELISA, Western Blot, Immunocytochemistry
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Synonyms
S100A8; Monoclonal Antibody; S100A8 (MRP-8); Anti -S100A8 (MRP-8); anti-S100A8 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
8H150
Specificity
Recognizes mouse S100A8.
Species Crossreactivity: Recombinant human S100A8: ~40%.
No crossreactivity is observed with recombinant mouse s100A4, recombinant mouse S100A9, recombinant mouse S100A10, recombinant mouse S100A11, recombinant human S100B or recombinant human S100P.
Species Crossreactivity: Recombinant human S100A8: ~40%.
No crossreactivity is observed with recombinant mouse s100A4, recombinant mouse S100A9, recombinant mouse S100A10, recombinant mouse S100A11, recombinant human S100B or recombinant human S100P.
Purity/Purification
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Concentration
~0.5 mg/ml (after reconstitution) (varies by lot)
Applicable Applications for anti-S100A8 antibody
ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
Western Blot: 2ug/ml using mouse leukocyte
Immunocytochemistry: 8-25 ug/ml using immersion fixed NIH-3T3 mouse embryonic fibroblast cell line
Optimal dilutions to be determined by the researcher.
Immunocytochemistry: 8-25 ug/ml using immersion fixed NIH-3T3 mouse embryonic fibroblast cell line
Optimal dilutions to be determined by the researcher.
Grade
Affinity Purified
Immunogen
Recombinant protein corresponding to Met1-Glu89 (predicted) from mouse S100A8, expressed in E. coli.
Preparation and Storage
Lyophilized and reconstituted products are stable for 12 months after receipt at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Application Data
(S100A8 in NIH-3T3 mouse cell line: S100A8 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using 10ug/mL AAA14670 for 3 hours at RT. Cells were stained (red) and counterstained with DAPI (blue).)
Application Data
(S100A8 in NIH-3T3 mouse cell line: S100A8 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using 10ug/mL AAA14670 for 3 hours at RT. Cells were stained (red) and counterstained with DAPI (blue).)
WB (Western Blot)
(Western Blot analysis of mouse S100A8 of lysates of mouse leukocyte. PVDF membrane was probed with AAA14670 (2ug/ml) followed by IgG (HRP) anti-rat IgG secondary antibody. A specific band was detected for S100A8 at ~10kD (as indicated). This experiment was conducted under reducing conditions and using the buffers listed in the protocol.)
WB (Western Blot)
(Western Blot analysis of mouse S100A8 of lysates of mouse leukocyte. PVDF membrane was probed with AAA14670 (2ug/ml) followed by IgG (HRP) anti-rat IgG secondary antibody. A specific band was detected for S100A8 at ~10kD (as indicated). This experiment was conducted under reducing conditions and using the buffers listed in the protocol.)
Related Product Information for anti-S100A8 antibody
Mouse S100A8 (also CP-10, calgranulin A and MRP8) is a 10kD member of the S100 family of calcium-binding proteins. The S100A8 protein is 89 amino acids (aa) in length and contains short, sequential modules. There is an N-terminal a-helix, followed by a calcium-binding EF-hand segment, and a C-terminal a-helix. S100A8 will noncovalently heterodimerize with S100A9. In the presence of calcium, this heterodimer will form a heterotetramer. The dimeric complex is both intracellular and extracellular. It binds heparan sulfate and is chemotactic for PMNs and macrophages. Mouse S100A8 is 80% and 57% aa identical to rat and human S100A8, respectively.
Product Categories/Family for anti-S100A8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
S100A8
NCBI Official Synonym Full Names
S100 calcium binding protein A8
NCBI Official Symbol
S100A8
NCBI Official Synonym Symbols
P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG; S100A8
NCBI Protein Information
protein S100-A8; MRP-8; calgranulin A; calgranulin-A; cystic fibrosis antigen; calprotectin L1L subunit; urinary stone protein band A; leukocyte L1 complex light chain; migration inhibitory factor-related protein 8; S100 calcium-binding protein A8 (calgranulin A)
UniProt Protein Name
Protein S100-A8
UniProt Gene Name
S100A8
UniProt Synonym Gene Names
CAGA; CFAG; MRP8
UniProt Entry Name
S10A8_HUMAN
Similar Products
Product Notes
The S100A8 s100a8 (Catalog #AAA14670) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The S100A8 (MRP-8) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's S100A8 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC). Western Blot: 2ug/ml using mouse leukocyte Immunocytochemistry: 8-25 ug/ml using immersion fixed NIH-3T3 mouse embryonic fibroblast cell line Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the S100A8 s100a8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S100A8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
