FCM (Flow Cytometry)
(T98G cells were stained with AAA14690 (filled histogram) or isotype control (open histogram).)
Mouse anti-Human Delta-like 1 Monoclonal Antibody
Mouse Anti-Delta-like 1
Reactivity
Human
Applications
Flow Cytometry
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Synonyms
Delta-like 1; Monoclonal Antibody; Mouse Anti-Delta-like 1; DLL1; Delta1; DL1; H-Delta-1; UNQ146/PRO172; anti-Delta-like 1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
9H109
Specificity
Recognizes human Delta-like 1. Does not crossreact with recombinant mouse DLL4.
Purity/Purification
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Form/Format
Supplied as a liquid in saline buffer, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Concentration
0.01 mg/ml (varies by lot)
Applicable Applications for anti-Delta-like 1 antibody
Flow Cytometry (FC)
Application Notes
FC: 10ul labels 1x10e6. T95G human Glioblastoma cell line.
Otimal dilutions to be determined by the researcher.
Other applications not tested.
Flow Cytometry:
1. T98G cells. may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, add 10ul of AAA14690 to <1x10e6 cells and incubated for 30 minutes at RT.
3. Wash the cells twice in Flow Cytometry Staining Buffer. Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.
4. Resuspended the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG2B antibody. This procedure may need to be modified, depending upon the cell type and final utilization. Individual users may need to titrate to determine the optimal reagent amount for their specific use.
Otimal dilutions to be determined by the researcher.
Other applications not tested.
Flow Cytometry:
1. T98G cells. may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, add 10ul of AAA14690 to <1x10e6 cells and incubated for 30 minutes at RT.
3. Wash the cells twice in Flow Cytometry Staining Buffer. Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.
4. Resuspended the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG2B antibody. This procedure may need to be modified, depending upon the cell type and final utilization. Individual users may need to titrate to determine the optimal reagent amount for their specific use.
Source
Human
Immunogen
Recombinant protein corresponding to Ser22-Gly540 of human Delta Like Protein 1 extracelular domain expressed in NSO cells.
Grade
Affinity Purified
Conjugation
APC
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
FCM (Flow Cytometry)
(T98G cells were stained with AAA14690 (filled histogram) or isotype control (open histogram).)
FCM (Flow Cytometry)
(T98G cells were stained with AAA14690 (filled histogram) or isotype control (open histogram).)
Related Product Information for anti-Delta-like 1 antibody
DLL1 is the human homolog of the Drosophila Notch ligand, Delta. It is a type I transmembrane protein with a DSL (Delta, Serrate, Jagged) domain followed by 8 EGF-like domains in its extracellular region and a short cytoplasmic region. Notch-DSL signaling plays an important role in the differentiation processes of many tissues.
Product Categories/Family for anti-Delta-like 1 antibody
NCBI and Uniprot Product Information
NCBI GeneID
Similar Products
Product Notes
The Delta-like 1 (Catalog #AAA14690) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Delta-like 1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Delta-like 1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC). FC: 10ul labels 1x10e6. T95G human Glioblastoma cell line. Otimal dilutions to be determined by the researcher. Other applications not tested. Flow Cytometry: 1. T98G cells. may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. 2. After blocking, add 10ul of AAA14690 to <1x10e6 cells and incubated for 30 minutes at RT. 3. Wash the cells twice in Flow Cytometry Staining Buffer. Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer. 4. Resuspended the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG2B antibody. This procedure may need to be modified, depending upon the cell type and final utilization. Individual users may need to titrate to determine the optimal reagent amount for their specific use. Researchers should empirically determine the suitability of the Delta-like 1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Delta-like 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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