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Standard Curve (Sample)

Rat actin, alpha 2, smooth muscle, aorta ELISA Kit | ACTA2 elisa kit

Rat alpha-Smooth muscle actin, alpha-SMA ELISA Kit

Reactivity
Rat
Synonyms
actin, alpha 2, smooth muscle, aorta; N/A; Rat alpha-Smooth muscle actin, alpha-SMA ELISA Kit; Rat alpha-Smooth muscle actin; alpha-SMA ELISA Kit; AAT6; ACTSA; alpha 2 actin; alpha-cardiac actin; growth-inhibiting gene 46; actin; alpha 2; smooth muscle; aorta; ACTA2 elisa kit
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For Research Use Only!
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat alpha-SMA. No significant cross-reactivity or interference between rat alpha-SMA and analogues was observed.
Sequence Length
377
Assay Type
Quantitative Sandwich
Samples
Serum, plasma and tissue homogenates
Detection Range
0.78 ng/ml-50 ng/ml
Sensitivity
< 0.195 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for ACTA2 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for alpha-SMA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any alpha-SMA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for alpha-SMA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of alpha-SMA bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42,009 Da
NCBI Official Full Name
actin, aortic smooth muscle
NCBI Official Symbol
ACTA2
NCBI Protein Information
actin, aortic smooth muscle; alpha-actin-2; alpha-smooth muscle actin
UniProt Protein Name
Actin, aortic smooth muscle
UniProt Gene Name
ACTA2
UniProt Synonym Gene Names
ACTSA; ACTVS
UniProt Entry Name
ACTA_RABIT

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Product Notes

The Rat ACTA2 acta2 (Catalog #AAA15073) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA15073 ELISA Kit recognizes Rat ACTA2. It is sometimes possible for the material contained within the vial of "actin, alpha 2, smooth muscle, aorta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.