Fish Acetylcholinesterase (AChE) ELISA Kit | AChE elisa kit

Fish Acetylcholinesterase, AChE ELISA Kit

Cat. Num. AAA15784

• Gene Names: ACHE; YT; ACEE; ARACHE; N-ACHE
• Reactivity: Fish
• Synonyms: Acetylcholinesterase (AChE); Fish Acetylcholinesterase; AChE ELISA Kit; Fish Acetylcholinesterase (AChE) ELISA Kit; ARACHE; N-ACHE; YT; OTTHUMP00000211347; OTTHUMP00000211349; OTTHUMP00000211356; acetylcholinesterase; apoptosis-related acetylcholinesterase; AChE elisa kit

• Reactivity: Fish
• Specificity: This assay has high sensitivity and excellent specificity for detection of fish AChE. No significant cross-reactivity or interference between fish AChE and analogues was observed.
• Sequence Length: 614
• Assay Type: Competitive
• Samples: Serum, plasma, tissue homogenates
• Detection Range: 2.5 ng/ml-40 ng/ml
• Sensitivity: < 2.5 ng/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for AChE elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to AChE. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated AChE. A competitive inhibition reaction is launched between AChE (Standards or samples) and Biotin-conjugated AChE with the pre-coated antibody specific for AChE. The more amount of AChE in samples, the less antibody bound by Biotin-conjugated AChE. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of AChE in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4557239
• NCBI GeneID: 43
• NCBI Accession #: NP_000656.1
• NCBI GenBank Nucleotide #: NM_000665.3
• UniProt Accession #: P22303; Q16169; Q29S23; Q2M324; Q504V3; Q53F46; Q86TM9; Q86YX9; Q9BXP7; A4D2E2; B7ZKZ0; D6W5X7
• Molecular Weight: 67,796 Da
• NCBI Official Full Name: acetylcholinesterase isoform E4-E6
• NCBI Official Synonym Full Names: acetylcholinesterase (Yt blood group)
• NCBI Official Symbol: ACHE
• NCBI Official Synonym Symbols: YT; ACEE; ARACHE; N-ACHE
• NCBI Protein Information: acetylcholinesterase; Yt blood group; apoptosis-related acetylcholinesterase
• UniProt Protein Name: Acetylcholinesterase
• UniProt Gene Name: ACHE
• UniProt Synonym Gene Names: AChE
• UniProt Entry Name: ACES_HUMAN