Standard Curve (Sample)
Rat Osteoprotegerin ELISA Kit | OPG elisa kit
Rat Osteoprotegerin ELISA Kit
Gene Names
TNFRSF11B; OPG; TR1; OCIF
Reactivity
Rat
Synonyms
Osteoprotegerin; Rat Osteoprotegerin ELISA Kit; OPG elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of OPG. No significant cross-reactivity or interference between OPG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between OPG and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive or Sandwich
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for OPG elisa kit
Intended Uses: This OPG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of RatOPG. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: OPG ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-OPG antibody and an OPG-HRP conjugate. The assay sample and buffer are incubated together with OPG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OPG concentration since OPG from samples and OPG-HRP conjugate compete for the anti-OPG antibody binding site. Since the number of sites is limited, as more sites are occupied by OPG from the sample, fewer sites are left to bind OPG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OPG concentration in each sample is interpolated from this standard curve.
Principle of the Assay: OPG ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-OPG antibody and an OPG-HRP conjugate. The assay sample and buffer are incubated together with OPG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OPG concentration since OPG from samples and OPG-HRP conjugate compete for the anti-OPG antibody binding site. Since the number of sites is limited, as more sites are occupied by OPG from the sample, fewer sites are left to bind OPG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OPG concentration in each sample is interpolated from this standard curve.
Product Categories/Family for OPG elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
46,026 Da
NCBI Official Full Name
osteoprotegerin
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 11b
NCBI Official Symbol
TNFRSF11B
NCBI Official Synonym Symbols
OPG; TR1; OCIF
NCBI Protein Information
tumor necrosis factor receptor superfamily member 11B; osteoprotegerin; osteoclastogenesis inhibitory factor
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 11B
UniProt Gene Name
TNFRSF11B
UniProt Synonym Gene Names
OCIF; OPG
UniProt Entry Name
TR11B_HUMAN
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Product Notes
The Rat OPG tnfrsf11b (Catalog #AAA16038) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA16038 ELISA Kit recognizes Rat OPG. It is sometimes possible for the material contained within the vial of "Osteoprotegerin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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