Rat Apoprotein B100 ELISA Kit | Apo-B100 elisa kit

Rat Apoprotein B100 ELISA Kit

Cat. Num. AAA16139

• Gene Names: APOB; FLDB; LDLCQ4
• Reactivity: Rat
• Synonyms: Apoprotein B100; Rat Apoprotein B100 ELISA Kit; Apo-B100 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of ApoB100. No significant cross-reactivity or interference between ApoB100 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ApoB100 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ?g/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for Apo-B100 elisa kit

Principle of the Assay: ApoB100 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ApoB100 antibody and an ApoB100-HRP conjugate. The assay sample and buffer are incubated together with ApoB100-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ApoB100 concentration since ApoB100 from samples and ApoB100-HRP conjugate compete for the anti-ApoB100 antibody binding site. Since the number of sites is limited, as more sites are occupied by ApoB100 from the sample, fewer sites are left to bind ApoB100-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ApoB100 concentration in each sample is interpolated from this standard curve.

Intended Uses: This ApoB100 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ApoB100. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for Apo-B100 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 28780
• NCBI GeneID: 338
• UniProt Accession #: P04114; O00502; P78479; P78480; P78481; Q13779; Q13785; Q13786; Q13787; Q13788; Q4ZG63; Q53QC8
• Molecular Weight: 515,605 Da
• NCBI Official Full Name: apo-B100
• NCBI Official Synonym Full Names: apolipoprotein B
• NCBI Official Symbol: APOB
• NCBI Official Synonym Symbols: FLDB; LDLCQ4
• NCBI Protein Information: apolipoprotein B-100; apoB-48; apoB-100; apo B-100; mutant Apo B 100; apolipoprotein B48; apolipoprotein B (including Ag(x) antigen)
• UniProt Protein Name: Apolipoprotein B-100
• UniProt Gene Name: APOB
• UniProt Synonym Gene Names: Apo B-100; Apo B-48
• UniProt Entry Name: APOB_HUMAN