Rat Glyceraldehyde 3 Phosphate Dehydrogenase ELISA Kit | GAPDH elisa kit

Rat Glyceraldehyde 3 Phosphate Dehydrogenase ELISA Kit

Cat. Num. AAA16397

• Gene Names: Gapdh; Gapd; BARS-38
• Reactivity: Rat
• Synonyms: Glyceraldehyde 3 Phosphate Dehydrogenase; N/A; Rat Glyceraldehyde 3 Phosphate Dehydrogenase ELISA Kit; GAPDH elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of GAPDH. No significant cross-reactivity or interference between GAPDH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GAPDH and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for GAPDH elisa kit

Principle of the Assay: GAPDH ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GAPDH antibody and an GAPDH-HRP conjugate. The assay sample and buffer are incubated together with GAPDH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GAPDH concentration since GAPDH from samples and GAPDH-HRP conjugate compete for the anti-GAPDH antibody binding site. Since the number of sites is limited, as more sites are occupied by GAPDH from the sample, fewer sites are left to bind GAPDH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAPDH concentration in each sample is interpolated from this standard curve.

Intended Uses: This GAPDH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat GAPDH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for GAPDH elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 122065190
• NCBI GeneID: 24383
• Molecular Weight: 35,828 Da
• NCBI Official Full Name: Glyceraldehyde-3-phosphate dehydrogenase
• NCBI Official Synonym Full Names: glyceraldehyde-3-phosphate dehydrogenase
• NCBI Official Symbol: Gapdh
• NCBI Official Synonym Symbols: Gapd; BARS-38
• NCBI Protein Information: glyceraldehyde-3-phosphate dehydrogenase; peptidyl-cysteine S-nitrosylase GAPDH; 38 kDa BFA-dependent ADP-ribosylation substrate
• UniProt Protein Name: Glyceraldehyde-3-phosphate dehydrogenase
• UniProt Gene Name: Gapdh
• UniProt Synonym Gene Names: Gapd; GAPDH
• UniProt Entry Name: G3P_RAT