Standard Curve (Sample)
Monkey Complement Component 5a ELISA Kit | C5a elisa kit
Monkey Complement Component 5a ELISA Kit
Gene Names
C5AR1; C5A; C5AR; C5R1; CD88
Reactivity
Monkey
Synonyms
Complement Component 5a; Monkey Complement Component 5a ELISA Kit; C5a elisa kit
Reactivity
Monkey
Specificity
Specifically recognize C5a, no obvious cross reaction with other analogues
Sequence Length
350
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for C5a elisa kit
Background: C5a is a small peptide molecule in the complement system, formed by the cleavage of complement C5, and has strong chemotactic activity. It is a critical regulatory molecule in inflammation and immune responses, capable of attracting various immune cells (such as neutrophils, monocytes, T cells, and macrophages) to the site of inflammation, and participating in the activation, aggregation, and mediation of immune cells and inflammatory responses. C5a promotes the chemotaxis and activation of immune cells by interacting with its specific receptor C5aR, inducing the activation of intracellular signaling pathways, ultimately resulting in various biological effects such as extracellular matrix degradation, intracellular signal transduction, cell apoptosis, and production of inflammatory mediators. In addition to its role in normal immune responses, excessive production and abnormal activation of C5a are also associated with various diseases such as autoimmune diseases, infectious diseases, cardiovascular diseases, neurological diseases, and cancer.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti C5a antibody was pre-coated onto the 96-well plate. The biotin conjugated anti C5a antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with C5a conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of C5a in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti C5a antibody was pre-coated onto the 96-well plate. The biotin conjugated anti C5a antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with C5a conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of C5a in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
39,336 Da
NCBI Official Full Name
complement component 5a receptor 1
NCBI Official Synonym Full Names
complement component 5a receptor 1
NCBI Official Symbol
C5AR1
NCBI Official Synonym Symbols
C5A; C5AR; C5R1; CD88
NCBI Protein Information
C5a anaphylatoxin chemotactic receptor 1
UniProt Protein Name
C5a anaphylatoxin chemotactic receptor 1
UniProt Gene Name
C5AR1
UniProt Synonym Gene Names
C5AR; C5R1; C5a-R; C5aR
UniProt Entry Name
C5AR1_HUMAN
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Product Notes
The Monkey C5a c5ar1 (Catalog #AAA17637) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA17637 ELISA Kit recognizes Monkey C5a. It is sometimes possible for the material contained within the vial of "Complement Component 5a, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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