Human Collagen Type IV ELISA Kit | COL4 elisa kit

Human Collagen Type IV ELISA Kit

Cat. Num. AAA17640

• Gene Names: Cg25C; alpha(IV)1/Cg25C; alpha1; cg25c; cg25C; CG25C; CG4145; Cgc25; Cgc25C; Col IV alpha(1); col4a1; Coll; Coll IV; CT12803; Dcg; Dcg1; DCg1; DmelCG4145; gp125
• Reactivity: Human
• Synonyms: Collagen Type IV; Human Collagen Type IV ELISA Kit; COL4/Type-IV Collagen; COL4 elisa kit

• Reactivity: Human
• Specificity: Specifically recognize COL4, no obvious cross reaction with other analogues
• Sequence Length: 1779
• Assay Type: Sandwich
• Samples: Serum, plasma, cell culture supernatant and other biological samples
• Detection Range: 0.781-50ng/ml
• Sensitivity: 0.469ng/ml

• Intra-assay Precision: Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
• Inter-assay Precision: Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for COL4 elisa kit

Background: Type IV collagen is the main collagen component of the basement membrane. It is a network-forming collagen that underlies epithelial and endothelial cells and functions as a barrier between tissue compartments. Type IV collagen has many binding partners and forms the backbone of the basement membrane. It holds important signaling potential as subdomains such as tumstatin are released when the protein is degraded by special proteases. Consequently, type IV collagen is both the most important structural collagen of the basement membrane and it entails key signaling potential, which is important for various physiological and pathological functions.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti COL4 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti COL4 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with COL4 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of COL4 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

NCBI and Uniprot Product Information

• NCBI GI #: 24581824
• NCBI GeneID: 33727
• NCBI Accession #: NP_723046.1
• NCBI GenBank Nucleotide #: NM_164617.2
• Molecular Weight: 174,300 Da
• NCBI Official Full Name: collagen type IV, isoform C
• NCBI Official Synonym Full Names: Collagen type IV
• NCBI Official Symbol: Cg25C
• NCBI Official Synonym Symbols: alpha(IV)1/Cg25C; alpha1; cg25c; cg25C; CG25C; CG4145; Cgc25; Cgc25C; Col IV alpha(1); col4a1; Coll; Coll IV; CT12803; Dcg; Dcg1; DCg1; DmelCG4145; gp125
• NCBI Protein Information: CG4145 gene product from transcript CG4145-RB
• UniProt Protein Name: Collagen alpha-1(IV) chain
• UniProt Gene Name: Cg25C
• UniProt Synonym Gene Names: DCg1
• UniProt Entry Name: CO4A1_DROME