IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Human Kidney Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human COL4a4 AntibodySecond Ab: 2ug/mL HRPLinked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog:))
Rabbit Collagen Type IV Alpha 4 Polyclonal Antibody | anti-COL4a4 antibody
Collagen Type IV Alpha 4 (COL4a4) Polyclonal Antibody
Gene Names
COL4A4; CA44
Reactivity
Homo sapiens (Human)
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Collagen Type IV Alpha 4; Polyclonal Antibody; Collagen Type IV Alpha 4 (COL4a4) Polyclonal Antibody; COL4-A4; CA44; Collagen Alpha-4(IV)chain; Collagen Of Basement Membrane; Alpha-4 Chain; anti-COL4a4 antibody
Host
Rabbit
Reactivity
Homo sapiens (Human)
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
0.45mg/ml (varies by lot)
Applicable Applications for anti-COL4a4 antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
Application Notes
Western blotting: 0.5-2ug/mL;
Immunohistochemistry: 5-20ug/mL;
Immunocytochemistry: 5-20ug/mL;
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;
Immunocytochemistry: 5-20ug/mL;
Optimal working dilutions must be determined by end user.
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant COL4a4 (Val1469~Ser1690) expressed in E.coli .
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Human Kidney Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human COL4a4 AntibodySecond Ab: 2ug/mL HRPLinked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog:))
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Human Kidney Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human COL4a4 AntibodySecond Ab: 2ug/mL HRPLinked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog:))
WB (Western Blot)
(Western Blot; Sample: Lane 1: 293T cell lysate; Lane 2: Hela cell lysatePrimary Ab: 2ug/ml Rabbit Anti-Human COL4a4 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog:))
WB (Western Blot)
(Western Blot; Sample: Lane 1: 293T cell lysate; Lane 2: Hela cell lysatePrimary Ab: 2ug/ml Rabbit Anti-Human COL4a4 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog:))
WB (Western Blot)
(Figure. Western Blot;Sample: Recombinant COL4a4, Human.)
WB (Western Blot)
(Figure. Western Blot;Sample: Recombinant COL4a4, Human.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
collagen alpha-4(IV) chain
NCBI Official Synonym Full Names
collagen type IV alpha 4 chain
NCBI Official Symbol
COL4A4
NCBI Official Synonym Symbols
CA44
NCBI Protein Information
collagen alpha-4(IV) chain
UniProt Protein Name
Collagen alpha-4(IV) chain
UniProt Gene Name
COL4A4
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Product Notes
The COL4a4 col4a4 (Catalog #AAA21020) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Collagen Type IV Alpha 4 (COL4a4) Polyclonal Antibody reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen Type IV Alpha 4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL; Immunohistochemistry: 5-20ug/mL; Immunocytochemistry: 5-20ug/mL; Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the COL4a4 col4a4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen Type IV Alpha 4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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