Human Tryptase ELISA Kit | TPS elisa kit

Human Tryptase ELISA Kit

Cat. Num. AAA17660

• Gene Names: TPSAB1; TPS1; TPS2; TPSB1
• Reactivity: Human
• Synonyms: Tryptase; Human Tryptase ELISA Kit; TPS; TPS elisa kit

• Reactivity: Human
• Specificity: Specifically recognize TPS, no obvious cross reaction with other analogues
• Sequence Length: 275
• Assay Type: Sandwich
• Samples: Serum, plasma, cell culture supernatant and other biological samples
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml

• Intra-assay Precision: Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
• Inter-assay Precision: Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for TPS elisa kit

Background: Tryptase is the most abundant neutral serine protease expressed in the secretory granules of all human mast cells. Tryptase is secreted upon the coupled activation-degranulation response of mast cells in peripheral tissues to physical factors such as trauma, toxins, venoms, endogenous mediators, and immune mechanisms (IgE-dependent and IgE-independent). Tryptase has distinct enzymatic functions that depend on the monomeric or homotetrameric state of this protein, the pH of the environment, and the presence or absence of heparin. Tryptase has the ability to cleave extracellular substrates such as vasoactive intestinal peptides, calcitonin gene-related peptides, fibronectins, fibrinogens, and kininogens. Tryptase is also a potent growth factor for epithelial cells, airway smooth muscle cells, and fibroblasts.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti TPS antibody was pre-coated onto the 96-well plate. The biotin conjugated anti TPS antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with TPS conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of TPS in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

NCBI and Uniprot Product Information

• NCBI GI #: 1182067
• NCBI GeneID: 7177
• Molecular Weight: 29,533 Da
• NCBI Official Full Name: tryptase
• NCBI Official Synonym Full Names: tryptase alpha/beta 1
• NCBI Official Symbol: TPSAB1
• NCBI Official Synonym Symbols: TPS1; TPS2; TPSB1
• NCBI Protein Information: tryptase alpha/beta-1
• UniProt Protein Name: Tryptase alpha/beta-1
• UniProt Gene Name: TPSAB1
• UniProt Synonym Gene Names: TPS1; TPS2; TPSB1; Tryptase-1
• UniProt Entry Name: TRYB1_HUMAN