Human Apolipoprotein A-I-binding protein ELISA Kit | APOA1BP elisa kit

Human Apolipoprotein A-I-binding protein ELISA Kit

Cat. Num. AAA17708

• Reactivity: Human
• Synonyms: Apolipoprotein A-I-binding protein; N/A; Human Apolipoprotein A-I-binding protein ELISA Kit; APOA1BP/AIBP/YJEFN1/YjeF_N1/AI-BP/YjeF N-terminal domain-containing protein 1/Apolipoprotein A-I-binding protein ()/NAD (P)HX epimerase; APOA1BP elisa kit

• Reactivity: Human
• Specificity: Specifically recognize APOA1BP, no obvious cross reaction with other analogues
• Sequence Length: 235
• Assay Type: Sandwich
• Samples: Serum, plasma, cell culture supernatant and other biological samples
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml

• Intra-assay Precision: Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
• Inter-assay Precision: Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for APOA1BP elisa kit

Background: Apolipoprotein A-I-binding protein, also known as AIBP, is a protein encoded by the APOA1BP gene. It interacts with apolipoprotein A-I (apoA-I), the major protein component of high-density lipoprotein (HDL) particles, and modulates its function. APOA1BP is involved in cholesterol metabolism and transport, particularly in the reverse cholesterol transport pathway, where it facilitates cholesterol efflux from peripheral tissues to the liver for excretion. APOA1BP has been implicated in the regulation of lipid metabolism, inflammation, and atherosclerosis. Studies have shown that APOA1BP expression is altered in various metabolic diseases, such as obesity, diabetes, and cardiovascular diseases, suggesting its potential as a therapeutic target for metabolic disorders.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti APOA1BP antibody was pre-coated onto the 96-well plate. The biotin conjugated anti APOA1BP antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with APOA1BP conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of APOA1BP in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

NCBI and Uniprot Product Information

• NCBI GI #: 318054060
• NCBI GeneID: 100528448
• NCBI Accession #: NP_001187647.1
• NCBI GenBank Nucleotide #: NM_001200718.1
• NCBI Official Full Name: apolipoprotein A-I-binding protein
• NCBI Official Symbol: aibp
• NCBI Protein Information: apolipoprotein A-I-binding protein