IHC (Immunohistochemistry)
(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Histone H2A (Phospho-Thr121) antibody.)
Rabbit Histone H2A Antibody | anti-HIST1H2AI antibody
Histone H2A (Phospho-Thr121) Antibody
Gene Names
HIST1H2AI; H2A/c; H2AFC
Reactivity
Human (Identities = 100%, Positives = 100%)Mouse (Identities = 100%, Positives = 100%)
Rat (Identities = 100%, Positives = 100%)
Applications
Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
Histone H2A; Histone H2A (Phospho-Thr121) Antibody; H2A.1b; H2A/c; H2A/d; H2A/i; H2A/n; H2A/p; H2A1; H2AC; H2AFC; H2AFD ANDHIST1H2AL; H2AFI; H2AFN ANDHIST1H2AG; H2AFP; HIST1H2AI; HIST1H2AJ; HIST1H2AM; Histone H2A.c/d/i/n/p; anti-HIST1H2AI antibody
Host
Rabbit
Reactivity
Human (Identities = 100%, Positives = 100%)
Mouse (Identities = 100%, Positives = 100%)
Rat (Identities = 100%, Positives = 100%)
Mouse (Identities = 100%, Positives = 100%)
Rat (Identities = 100%, Positives = 100%)
Isotype
IgG
Specificity
Histone H2A (Phospho-Thr121) antibody detects endogenous levels of Histone H2A only when phosphorylated at threonine 121.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
130
Applicable Applications for anti-HIST1H2AI antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
IHC: 1:50~1:100
ELISA: 1:1000
ELISA: 1:1000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Histone H2A around the phosphorylation site of threonine 121 (K-K-TP-E-S).
All Sites
Human: Thr121
Mouse: Thr121
Rat: Thr121
Mouse: Thr121
Rat: Thr121
Preparation and Storage
Store at -20 degree C for 1 year.
IHC (Immunohistochemistry)
(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Histone H2A (Phospho-Thr121) antibody.)
IHC (Immunohistochemistry)
(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Histone H2A (Phospho-Thr121) antibody.)
Product Categories/Family for anti-HIST1H2AI antibody
References
Albig W., Hum. Genet. 101:284-294(1997). Albig W., Biol. Chem. 380:7-18(1999). Dobner T., DNA Seq. 1:409-413(1991).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
histone H2A type 1
NCBI Official Synonym Full Names
histone cluster 1 H2A family member i
NCBI Official Symbol
HIST1H2AI
NCBI Official Synonym Symbols
H2A/c; H2AFC
NCBI Protein Information
histone H2A type 1
UniProt Protein Name
Histone H2A type 1
UniProt Gene Name
HIST1H2AG
UniProt Synonym Gene Names
H2AFP; H2A.1
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Product Notes
The HIST1H2AI hist1h2ag (Catalog #AAA17961) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2A (Phospho-Thr121) Antibody reacts with Human (Identities = 100%, Positives = 100%) Mouse (Identities = 100%, Positives = 100%) Rat (Identities = 100%, Positives = 100%) and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2A can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA). IHC: 1:50~1:100 ELISA: 1:1000. Researchers should empirically determine the suitability of the HIST1H2AI hist1h2ag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2A, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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