Mouse meteorin, glial cell differentiation regulator-like ELISA Kit | METRNL elisa kit
Mouse Meteorin-like protein, METRNL ELISA Kit
Gene Names
Metrnl; BC019776; 9430048M07Rik
Reactivity
Mouse
Synonyms
meteorin, glial cell differentiation regulator-like; N/A; Mouse Meteorin-like protein, METRNL ELISA Kit; Mouse Meteorin-like protein (METRNL) ELISA kit; MGC99788; ; meteorin; glial cell differentiation regulator-like; METRNL elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse METRNL. No significant cross-reactivity or interference between mouse METRNL and analogues was observed.
Sequence Length
311
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
31.25 pg/ml -2000 pg/ml.
Sensitivity
The minimum detectable dose of mouse METRNL is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for METRNL elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for METRNL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any METRNL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for METRNL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of METRNL bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,530 Da
NCBI Official Full Name
meteorin-like protein
NCBI Official Synonym Full Names
meteorin, glial cell differentiation regulator-like
NCBI Official Symbol
Metrnl
NCBI Official Synonym Symbols
BC019776; 9430048M07Rik
NCBI Protein Information
meteorin-like protein; cometin
UniProt Protein Name
Meteorin-like protein
UniProt Gene Name
Metrnl
UniProt Entry Name
METRL_MOUSE