Human Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4) ELISA Kit | TNFSF4 elisa kit
Human Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4) ELISA Kit
Gene Names
TNFSF4; GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L
Reactivity
Human
Synonyms
Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4); N/A; Human Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4) ELISA Kit; CD252; TXGP1; OX40L; Gp34; OX40-L; Tax-Transcriptionally Activated Glycoprotein 1,34kD; Glycoprotein Gp34; OX40 ligand; TNFSF4 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of TNFSF4. No significant cross-reactivity or interference between TNFSF4 and analogues was observed.
Sequence Length
133
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Detection Range
15.6-1,000pg/mL
Sensitivity
6.0pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNFSF4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNFSF4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Related Product Information for TNFSF4 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TNFSF4 in human serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TNFSF4. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TNFSF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNFSF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TNFSF4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TNFSF4. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TNFSF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNFSF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TNFSF4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for TNFSF4 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,427 Da
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 4 isoform 1
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 4
NCBI Official Symbol
TNFSF4
NCBI Official Synonym Symbols
GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L
NCBI Protein Information
tumor necrosis factor ligand superfamily member 4
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 4
UniProt Gene Name
TNFSF4
UniProt Synonym Gene Names
TXGP1; OX40L
UniProt Entry Name
TNFL4_HUMAN
