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IHC (Immunohistchemistry)
(DAB staining on IHC-P;Samples: Mouse Testis Tissue.)
Mouse Brain Natriuretic Peptide (BNP) Monoclonal Antibody | anti-BNP antibody
Monoclonal Antibody to Brain Natriuretic Peptide (BNP)
Gene Names
Nppb; BNF; BNP; AA408272
Reactivity
Mus musculus (Mouse)
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Protein A + Protein G affinity chromatography
Synonyms
Brain Natriuretic Peptide (BNP); Monoclonal Antibody; Monoclonal Antibody to Brain Natriuretic Peptide (BNP); GC-B; NPPB; B-Type Natriuretic Peptide; Natriuretic Peptide Precursor B; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32; anti-BNP antibody
Host
Mouse
Reactivity
Mus musculus (Mouse)
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
C10
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-BNP antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Source
Monoclonal antibody preparation
Traits
Liquid
Cross Reactivity
Human; Rat
Immunogen
Recombinant BNP (Tyr27~Leu121) expressed in E.coli .
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
IHC (Immunohistchemistry)
(DAB staining on IHC-P;Samples: Mouse Testis Tissue.)
IHC (Immunohistchemistry)
(DAB staining on IHC-P;Samples: Mouse Testis Tissue.)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Mouse Liver Tissue)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Mouse Liver Tissue)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Mouse Kidney Tissue)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Mouse Kidney Tissue)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Mouse Brain Tissue.)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Mouse Brain Tissue.)
WB (Western Blot)
(Western Blot:Sample:Lane 1: Rat Heart Tissue;Lane 2: Rat Testis Tissue.)
WB (Western Blot)
(Western Blot:Sample:Lane 1: Rat Heart Tissue;Lane 2: Rat Testis Tissue.)
WB (Western Blot)
(Western Blot:Sample: Recombinant BNP, Mouse.)
WB (Western Blot)
(Western Blot:Sample: Recombinant BNP, Mouse.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
natriuretic peptides B isoform 2 preproprotein
NCBI Official Synonym Full Names
natriuretic peptide type B
NCBI Official Symbol
Nppb
NCBI Official Synonym Symbols
BNF; BNP; AA408272
NCBI Protein Information
natriuretic peptides B
UniProt Protein Name
Natriuretic peptides B
UniProt Gene Name
Nppb
UniProt Synonym Gene Names
BNP
Similar Products
Product Notes
The BNP nppb (Catalog #AAA21050) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Brain Natriuretic Peptide (BNP) reacts with Mus musculus (Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Brain Natriuretic Peptide (BNP) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the BNP nppb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Brain Natriuretic Peptide (BNP), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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