<
IHC (Immunohistochemistry)
(CD276/B7-H3 Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit anti-Human CD276/B7-H3 Polyclonal Antibody | anti-CD276 antibody
CD276/B7-H3 Rabbit anti-Human Polyclonal (aa47-219) Antibody
Gene Names
CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
CD276/B7-H3; Polyclonal Antibody; CD276/B7-H3 Rabbit anti-Human Polyclonal (aa47-219) Antibody; PathPlus CD276/B7-H3 Antibody (aa47-219); CD276; 4Ig-B7-H3; B7 homolog 3; B7-H3; CD276 antigen; CD276 molecule; Costimulatory molecule; B7H3; B7RP-2; anti-CD276 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CD276. This antibody hase been validated against CD276 knockout 293T cells.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
PBS, pH7.4, 0.02% Sodium Azide, 50% Glycerol
Concentration
0.5mg/ml (varies by lot)
Applicable Applications for anti-CD276 antibody
Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
ICC: 5-20ug/ml
IHC-P: 10ug/ml
WB: 0.5-2ug/ml
ELISA: 0.05-2ug/ml
IHC-P: 10ug/ml
WB: 0.5-2ug/ml
ELISA: 0.05-2ug/ml
Target
Human CD276/B7-H3
Immunogen
Recombinant CD276 (Thr47-Ser219) expressed in E Coli.
Conjugation
Unconjugated
Epitope
aa47-219
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
IHC (Immunohistochemistry)
(CD276/B7-H3 Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(CD276/B7-H3 Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistchemistry)
(CD276/B7-H3 Antibody-Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistchemistry)
(CD276/B7-H3 Antibody-Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(CD276/B7-H3 Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(CD276/B7-H3 Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(CD276/B7-H3 Antibody-Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(CD276/B7-H3 Antibody-Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot)
(CD276/B7-H3 Antibody-Western blot of recombinant CD276/B7-H3.)
WB (Western Blot)
(CD276/B7-H3 Antibody-Western blot of recombinant CD276/B7-H3.)
WB (Western Blot)
(CD276/B7-H3 Antibody-Western Blot; Sample: Lane1: Human 293T Cells; Lane2: Human PC-3 Cells; Lane3: Human MCF-7 Cells.)
WB (Western Blot)
(CD276/B7-H3 Antibody-Western Blot; Sample: Lane1: Human 293T Cells; Lane2: Human PC-3 Cells; Lane3: Human MCF-7 Cells.)
WB (Western Blot)
(CD276/B7-H3 Antibody-Knockout Varification: Lane 1: Wild-type 293T cell lysate; Lane 2: CD276 knockout 293T cell lysate; Predicted MW: 57,53,33kd Observed MW: 100kd Primary Ab: 3ug/ml Rabbit Anti-Human CD276 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(CD276/B7-H3 Antibody-Knockout Varification: Lane 1: Wild-type 293T cell lysate; Lane 2: CD276 knockout 293T cell lysate; Predicted MW: 57,53,33kd Observed MW: 100kd Primary Ab: 3ug/ml Rabbit Anti-Human CD276 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Related Product Information for anti-CD276 antibody
B7-H3 antibody is an unconjugated rabbit polyclonal antibody to human B7-H3 (CD276)(aa47-219). Validated for ICC, IHC, IP and WB.
CD276 (B7-H3) is a type I transmembrane protein and immune checkpoint inhibitor that is induced on antigen presenting cells. It plays a role in the inhibition of T-cell function. It is expressed constitutively on fibroblasts, endothelial cells, and osteoblasts. It is also induced on immune cells, particularly antigen-presenting dendritic cells, monocytes, NK cells, B cells, and some T-cells. It is overexpressed in a range of human cancers, including melanomas, leukemia, breast, prostate, ovarian, pancreatic, and colorectal cancers, while limited expression is seen in normal tissues. Overexpression of CD276 correlates with poor prognosis, suggesting that CD276 plays a role in immune evasion and tumorigenesis. It is a target for cancer immunotherapy and a candidate for inhibitory immune checkpoint blockade.
CD276 (B7-H3) is a type I transmembrane protein and immune checkpoint inhibitor that is induced on antigen presenting cells. It plays a role in the inhibition of T-cell function. It is expressed constitutively on fibroblasts, endothelial cells, and osteoblasts. It is also induced on immune cells, particularly antigen-presenting dendritic cells, monocytes, NK cells, B cells, and some T-cells. It is overexpressed in a range of human cancers, including melanomas, leukemia, breast, prostate, ovarian, pancreatic, and colorectal cancers, while limited expression is seen in normal tissues. Overexpression of CD276 correlates with poor prognosis, suggesting that CD276 plays a role in immune evasion and tumorigenesis. It is a target for cancer immunotherapy and a candidate for inhibitory immune checkpoint blockade.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,165 Da
NCBI Official Full Name
CD276 antigen isoform b
NCBI Official Synonym Full Names
CD276 molecule
NCBI Official Symbol
CD276
NCBI Official Synonym Symbols
B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
NCBI Protein Information
CD276 antigen; B7 homolog 3; costimulatory molecule
UniProt Protein Name
CD276 antigen
UniProt Gene Name
CD276
UniProt Synonym Gene Names
B7H3; B7-H3
UniProt Entry Name
CD276_HUMAN
Similar Products
Product Notes
The CD276 cd276 (Catalog #AAA21333) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD276/B7-H3 Rabbit anti-Human Polyclonal (aa47-219) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD276/B7-H3 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), Western Blot (WB). ICC: 5-20ug/ml IHC-P: 10ug/ml WB: 0.5-2ug/ml ELISA: 0.05-2ug/ml. Researchers should empirically determine the suitability of the CD276 cd276 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD276/B7-H3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
