<
IHC (Immunohistchemistry)
(TIGIT Antibody-Immunocytochemistry of TIGIT in over expressing HEK293 cells using TIGIT antibody and control mouse IgG antibody (left corner box) at 1 ug/ml.)
Mouse anti-Human TIGIT Monoclonal Antibody | anti-TIGIT antibody
TIGIT Mouse anti-Human Monoclonal (3B5) Antibody
Gene Names
TIGIT; VSIG9; VSTM3; WUCAM
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
Protein A purified
Synonyms
TIGIT; Monoclonal Antibody; TIGIT Mouse anti-Human Monoclonal (3B5) Antibody; IHC-plus TIGIT Antibody (clone 3B5); VSTM3; WUCAM; VSIG9; anti-TIGIT antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
3B5
Purity/Purification
Protein A purified
Form/Format
PBS, 0.02% Sodium Azide, 50% Glycerol
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-TIGIT antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
IHC-P: 10ug/ml
TIGIT antibody can be used for immunohistochemistry starting at 2ug/mL. For immunofluorescence start at 1ug/mL. For flow cytometry at 1ug/ml. For immunocytochemistry at 1ug/mL. Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested. Western Blot: Predicted: 26 kDa Observed: 47 kDa
TIGIT antibody can be used for immunohistochemistry starting at 2ug/mL. For immunofluorescence start at 1ug/mL. For flow cytometry at 1ug/ml. For immunocytochemistry at 1ug/mL. Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested. Western Blot: Predicted: 26 kDa Observed: 47 kDa
Target
Human TIGIT
Immunogen
The extracellular domain of human TIGIT
Conjugation
Unconjugated
Family
New
Preparation and Storage
Store at 4 degree C for up 3 months. Store at -20 degree C for up to 1 year. Avoid freeze/thaw cycles.
IHC (Immunohistchemistry)
(TIGIT Antibody-Immunocytochemistry of TIGIT in over expressing HEK293 cells using TIGIT antibody and control mouse IgG antibody (left corner box) at 1 ug/ml.)
IHC (Immunohistchemistry)
(TIGIT Antibody-Immunocytochemistry of TIGIT in over expressing HEK293 cells using TIGIT antibody and control mouse IgG antibody (left corner box) at 1 ug/ml.)
IHC (Immunohistochemistry)
(TIGIT Antibody-Immunohistochemistry of TIGIT in human stomach carcinoma tissue using TIGIT Antibody and control mouse IgG (corner box) at 2 ug/ml.)
IHC (Immunohistochemistry)
(TIGIT Antibody-Immunohistochemistry of TIGIT in human stomach carcinoma tissue using TIGIT Antibody and control mouse IgG (corner box) at 2 ug/ml.)
IHC (Immunohistochemistry)
(TIGIT Antibody-Human Small Intestine, MALT: Formalin-Fixed, Paraffin-Embedded (FFPE) at 10 ug/ml.)
IHC (Immunohistochemistry)
(TIGIT Antibody-Human Small Intestine, MALT: Formalin-Fixed, Paraffin-Embedded (FFPE) at 10 ug/ml.)
ICC (Immunocytochemistry)
(TIGIT Antibody-Immunofluorescence of TIGIT in human stomach carcinoma tissue using TIGIT Antibody at 5 ug/ml. Green: TIGIT Antibody [3B5] Blue: DAPI staining)
ICC (Immunocytochemistry)
(TIGIT Antibody-Immunofluorescence of TIGIT in human stomach carcinoma tissue using TIGIT Antibody at 5 ug/ml. Green: TIGIT Antibody [3B5] Blue: DAPI staining)
ICC (Immunocytochemistry)
(TIGIT Antibody-Immunofluorescence of TIGIT in over expressing HEK293 cells using TIGIT Antibody at 1 ug/ml. Green: TIGIT Antibody [3B5] Blue: DAPI staining)
ICC (Immunocytochemistry)
(TIGIT Antibody-Immunofluorescence of TIGIT in over expressing HEK293 cells using TIGIT Antibody at 1 ug/ml. Green: TIGIT Antibody [3B5] Blue: DAPI staining)
FCM (Flow Cytometry)
(TIGIT Antibody-Flow cytometry analysis of TIGIT over expressing HEK293 cells using TIGIT antibody at 1 ug/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.)
FCM (Flow Cytometry)
(TIGIT Antibody-Flow cytometry analysis of TIGIT over expressing HEK293 cells using TIGIT antibody at 1 ug/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.)
Related Product Information for anti-TIGIT antibody
TIGIT antibody is an unconjugated mouse monoclonal antibody to human TIGIT. Validated for ELISA, Flow, ICC, IF and IHC. Tested on 20 paraffin-embedded human tissues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
18,432 Da
NCBI Official Full Name
T-cell immunoreceptor with Ig and ITIM domains
NCBI Official Synonym Full Names
T cell immunoreceptor with Ig and ITIM domains
NCBI Official Symbol
TIGIT
NCBI Official Synonym Symbols
VSIG9; VSTM3; WUCAM
NCBI Protein Information
T-cell immunoreceptor with Ig and ITIM domains
UniProt Protein Name
T-cell immunoreceptor with Ig and ITIM domains
UniProt Gene Name
TIGIT
UniProt Synonym Gene Names
VSIG9; VSTM3
Similar Products
Product Notes
The TIGIT tigit (Catalog #AAA21343) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TIGIT Mouse anti-Human Monoclonal (3B5) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TIGIT can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P). IHC-P: 10ug/ml TIGIT antibody can be used for immunohistochemistry starting at 2ug/mL. For immunofluorescence start at 1ug/mL. For flow cytometry at 1ug/ml. For immunocytochemistry at 1ug/mL. Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested. Western Blot: Predicted: 26 kDa Observed: 47 kDa. Researchers should empirically determine the suitability of the TIGIT tigit for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIGIT, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
