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IHC (Immunohistochemistry)
(CANX/Calnexin Antibody-Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit CANX/Calnexin Polyclonal Antibody | anti-CANX antibody
CANX/Calnexin Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Gene Names
CANX; CNX; P90; IP90
Reactivity
Mouse, Dog, Bovine, Rat, Human
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Immunoaffinity purified
Synonyms
CANX/Calnexin; Polyclonal Antibody; CANX/Calnexin Rabbit anti-Human Polyclonal (C-Terminus) Antibody; PathPlus CANX/Calnexin Antibody (C-Terminus); CANX; CNX; Calnexin; IP90; p90; anti-CANX antibody
Host
Rabbit
Reactivity
Mouse, Dog, Bovine, Rat, Human
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of Calnexin protein.
Purity/Purification
Immunoaffinity purified
Form/Format
PBS, pH7.3, 0.01% Sodium Azide, 30% Glycerol
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CANX antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), Western Blot (WB)
Target
Human CANX/Calnexin
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human Calnexin.
Conjugation
Unconjugated
Epitope
C-Terminus
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.
IHC (Immunohistochemistry)
(CANX/Calnexin Antibody-Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(CANX/Calnexin Antibody-Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistchemistry)
(CANX/Calnexin Antibody-Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistchemistry)
(CANX/Calnexin Antibody-Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(CANX/Calnexin Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(CANX/Calnexin Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(CANX/Calnexin Antibody-Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(CANX/Calnexin Antibody-Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(CANX/Calnexin Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(CANX/Calnexin Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot)
(CANX/Calnexin Antibody-Western blot analysis of Calnexin expression in MCF7 (A); HeLa (B); MDAMB231 (C) whole cell lysates.)
WB (Western Blot)
(CANX/Calnexin Antibody-Western blot analysis of Calnexin expression in MCF7 (A); HeLa (B); MDAMB231 (C) whole cell lysates.)
ICC (Immunocytochemistry)
(CANX/Calnexin Antibody-Immunofluorescent analysis of Calnexin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
ICC (Immunocytochemistry)
(CANX/Calnexin Antibody-Immunofluorescent analysis of Calnexin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Related Product Information for anti-CANX antibody
Calnexin antibody is an unconjugated rabbit polyclonal antibody to Calnexin (CANX)(C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC, IP and WB.
CANX (also called calnexin) is a calcium-binding molecular chaperone protein that is involved in facilitating the assembly and folding of newly synthesized N-linked glycoproteins. It is a stress-induced protein located in the endoplasmic reticulum membrane. It is also found in melanosomes, where it appears to be involved in melanosome biogenesis. CANX is broadly expressed in normal and diseased tissues, and expression may be increased or decreased in certain types of cancers.
CANX (also called calnexin) is a calcium-binding molecular chaperone protein that is involved in facilitating the assembly and folding of newly synthesized N-linked glycoproteins. It is a stress-induced protein located in the endoplasmic reticulum membrane. It is also found in melanosomes, where it appears to be involved in melanosome biogenesis. CANX is broadly expressed in normal and diseased tissues, and expression may be increased or decreased in certain types of cancers.
References
EMBO J. 2012;31(7):1823-1835. doi: 10.1038/emboj.2012.15; J Proteome Res. 2003;2(1):69-79. PMID: 12643545; J Transl Med. 2016;14(1):196. doi: 10.1186/s12967-016-0948-z; Cancer Lett. 2004;203(2):225-231. PMID: 14732231; Lung Cancer. 2015;90(2):342-345. doi: 10.1016/j.lungcan.2015.08.015; J Cell Mol Med. 2017;21(12):3141-3149. doi: 10.1111/jcmm.13413.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,598 Da
NCBI Official Full Name
calnexin
NCBI Official Synonym Full Names
calnexin
NCBI Official Symbol
CANX
NCBI Official Synonym Symbols
CNX; P90; IP90
NCBI Protein Information
calnexin; major histocompatibility complex class I antigen-binding protein p88
UniProt Protein Name
Calnexin
UniProt Gene Name
CANX
UniProt Entry Name
CALX_HUMAN
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Product Notes
The CANX canx (Catalog #AAA21353) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CANX/Calnexin Rabbit anti-Human Polyclonal (C-Terminus) Antibody reacts with Mouse, Dog, Bovine, Rat, Human and may cross-react with other species as described in the data sheet. AAA Biotech's CANX/Calnexin can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), Western Blot (WB). Researchers should empirically determine the suitability of the CANX canx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CANX/Calnexin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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