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IHC (Immunohistchemistry)
(HSPA5/GRP78/BiP Antibody-Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))
Goat anti-Human HSPA5/GRP78/BiP Polyclonal Antibody | anti-HSPA5 antibody
HSPA5/GRP78/BiP Goat anti-Human Polyclonal Antibody
Gene Names
HSPA5; BIP; MIF2; GRP78
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
HSPA5/GRP78/BiP; Polyclonal Antibody; HSPA5/GRP78/BiP Goat anti-Human Polyclonal Antibody; IHC-plus HSPA5/GRP78/BiP Antibody; HSPA5; BIP; Heat shock 70 kDa protein 5; MIF2; GRP-78; GRP78; anti-HSPA5 antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
TBS, pH7.3, 0.02% Sodium Azide, 0.5% BSA
Concentration
0.5mg/ml (varies by lot)
Applicable Applications for anti-HSPA5 antibody
Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Peptide-ELISA (EIA), Western Blot (WB)
Target
Human HSPA5/GRP78/BiP
Immunogen
Peptide with sequence C-ESHQDADIEDFK, from the internal region (near C-Terminus) of the protein sequence according to NP_005338.1.
Conjugation
Unconjugated
Preparation and Storage
Aliquot and store at -20 degree C. Avoid freeze-thaw cycles.
IHC (Immunohistchemistry)
(HSPA5/GRP78/BiP Antibody-Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistchemistry)
(HSPA5/GRP78/BiP Antibody-Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(HSPA5/GRP78/BiP Antibody-Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(HSPA5/GRP78/BiP Antibody-Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(HSPA5/GRP78/BiP Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(HSPA5/GRP78/BiP Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot)
(HSPA5/GRP78/BiP Antibody-HSPA5/GRP78/BiP antibody (0.3ug/ml) staining of Mouse Liver (A) and Testes (B) and (0.5ug/ml) Rat Brain (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
WB (Western Blot)
(HSPA5/GRP78/BiP Antibody-HSPA5/GRP78/BiP antibody (0.3ug/ml) staining of Mouse Liver (A) and Testes (B) and (0.5ug/ml) Rat Brain (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
WB (Western Blot)
(HSPA5/GRP78/BiP Antibody-HSPA5/GRP78/BiP antibody (1ug/ml) staining of HepG2 (A) and MCF7 (B) lysates (35ug protein in RIPA buffer).Detected by chemiluminescence.)
WB (Western Blot)
(HSPA5/GRP78/BiP Antibody-HSPA5/GRP78/BiP antibody (1ug/ml) staining of HepG2 (A) and MCF7 (B) lysates (35ug protein in RIPA buffer).Detected by chemiluminescence.)
ICC (Immunocytochemistry)
(HSPA5/GRP78/BiP Antibody-HSPA5/GRP78/BiP antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
ICC (Immunocytochemistry)
(HSPA5/GRP78/BiP Antibody-HSPA5/GRP78/BiP antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
Related Product Information for anti-HSPA5 antibody
BiP antibody is an unconjugated goat polyclonal antibody to human BiP (HSPA5/GRP78). Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
72,333 Da
NCBI Official Full Name
GRP78, partial
NCBI Official Synonym Full Names
heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
NCBI Official Symbol
HSPA5
NCBI Official Synonym Symbols
BIP; MIF2; GRP78
NCBI Protein Information
78 kDa glucose-regulated protein; immunoglobulin heavy chain-binding protein; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
UniProt Protein Name
78 kDa glucose-regulated protein
UniProt Gene Name
HSPA5
UniProt Synonym Gene Names
GRP78; GRP-78; BiP
UniProt Entry Name
GRP78_HUMAN
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Product Notes
The HSPA5 hspa5 (Catalog #AAA21379) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The HSPA5/GRP78/BiP Goat anti-Human Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSPA5/GRP78/BiP can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Peptide-ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the HSPA5 hspa5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSPA5/GRP78/BiP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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