Standard Curve (Sample)
Mouse GDNF ELISA Kit | GDNF elisa kit
Mouse GDNF (Glial Cell Line Derived Neurotrophic Factor) ELISA Kit
Gene Names
GDNF; ATF1; ATF2; HSCR3; HFB1-GDNF
Reactivity
Mouse
Synonyms
GDNF; Mouse GDNF (Glial Cell Line Derived Neurotrophic Factor) ELISA Kit; GDNF elisa kit
Reactivity
Mouse
Specificity
This kit recognizes Mouse GDNF in samples. No significant cross-reactivity or interference between Mouse GDNF and analogues was observed.
Sequence Length
202
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
15.63-1000pg/mL
Sensitivity
9.38pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse GDNF were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse GDNF were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for GDNF elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse GDNF concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GDNF. Samples (or Standards) and biotinylated detection antibody specific for Mouse GDNF are added to the micro ELISA plate wells. Mouse GDNF would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 +/- 2 nm. The OD value is proportional to the concentration of Mouse GDNF. You can calculate the concentration of Mouse GDNF in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GDNF. Samples (or Standards) and biotinylated detection antibody specific for Mouse GDNF are added to the micro ELISA plate wells. Mouse GDNF would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 +/- 2 nm. The OD value is proportional to the concentration of Mouse GDNF. You can calculate the concentration of Mouse GDNF in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
18,123 Da
NCBI Official Full Name
glial cell line-derived neurotrophic factor isoform 4 preproprotein
NCBI Official Synonym Full Names
glial cell derived neurotrophic factor
NCBI Official Symbol
GDNF
NCBI Official Synonym Symbols
ATF1; ATF2; HSCR3; HFB1-GDNF
NCBI Protein Information
glial cell line-derived neurotrophic factor; ATF; astrocyte-derived trophic factor
UniProt Protein Name
Glial cell line-derived neurotrophic factor
UniProt Gene Name
GDNF
UniProt Synonym Gene Names
hGDNF; ATF
UniProt Entry Name
GDNF_HUMAN
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Product Notes
The Mouse GDNF gdnf (Catalog #AAA21656) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA21656 ELISA Kit recognizes Mouse GDNF. It is sometimes possible for the material contained within the vial of "GDNF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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