WB (Western Blot)
(Detection of mouse PBRM1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 and TCMK-1 prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBRM1 antibody AAA23777 (lot AAA23777-5) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human, Mouse PBRM1 Polyclonal Antibody | anti-PBRM1 antibody
Rabbit anti-PBRM1 Antibody, Affinity Purified
IP: 6ug/mg lysate
IHC: 1:1000 to 1:5000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse PBRM1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 and TCMK-1 prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBRM1 antibody AAA23777 (lot AAA23777-5) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of mouse PBRM1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 and TCMK-1 prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBRM1 antibody AAA23777 (lot AAA23777-5) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human PBRM1 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBRM1 antibody AAA23777 (lot AAA23777-5) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human PBRM1 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBRM1 antibody AAA23777 (lot AAA23777-5) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human PBRM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PBRM1 antibody AAA23777 (lot AAA23777-5) used for IP at 6 ug per reaction. PBRM1 was also immunoprecipitated by a previous lot of this antibody (lot AAA23777-4) and rabbit anti-PBRM1 recombinant monoclonal antibody For blotting immunoprecipitated PBRM1, was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human PBRM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PBRM1 antibody AAA23777 (lot AAA23777-5) used for IP at 6 ug per reaction. PBRM1 was also immunoprecipitated by a previous lot of this antibody (lot AAA23777-4) and rabbit anti-PBRM1 recombinant monoclonal antibody For blotting immunoprecipitated PBRM1, was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of mouse PBRM1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-PBRM1 (Cat. No. AAA23777 Lot 5) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB.)
IHC (Immunohistochemistry)
(Detection of mouse PBRM1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-PBRM1 (Cat. No. AAA23777 Lot 5) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB.)
IHC (Immunohistochemistry)
(Detection of human PBRM1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PBRM1 (Cat. No. AAA23777 Lot 5) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB.)
IHC (Immunohistochemistry)
(Detection of human PBRM1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PBRM1 (Cat. No. AAA23777 Lot 5) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB.)
FCM (Flow Cytometry)
(Detection of human PBRM1 (shaded) in Ramos cells by flow cytometry. Antibody: Rabbit anti-PBRM1 polyclonal antibody (AAA23777) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human PBRM1 (shaded) in Ramos cells by flow cytometry. Antibody: Rabbit anti-PBRM1 polyclonal antibody (AAA23777) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
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Product Notes
The PBRM1 pbrm1 (Catalog #AAA23777) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PBRM1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PBRM1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). WB: 1:2000-1:10,000 IP: 6ug/mg lysate IHC: 1:1000 to 1:5000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Researchers should empirically determine the suitability of the PBRM1 pbrm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PBRM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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