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WB (Western Blot)
(Detection of human and mouse ICLN by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ICLN antibody AAA23778 (lot AAA23778-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse ICLN Polyclonal Antibody | anti-ICLN antibody
Rabbit anti-ICLN Antibody, Affinity Purified
Gene Names
CLNS1A; CLCI; ICln; CLNS1B
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Antigen Affinity Purified
Synonyms
ICLN, Antibody; Rabbit anti-ICLN Antibody, Affinity Purified; CLNS1B; ICln; reticulocyte protein ICln; methylosome subunit pICln; i(Cln); chloride ion current inducer protein; chloride channel regulatory protein; chloride conductance regulatory protein ICln; reticulocyte pICln; chloride channel, nucleotide sensitive 1A; CLCI; Methylosome subunit pICln; chloride nucleotide-sensitive channel 1A; anti-ICLN antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ICLN antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:1000-1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.25 ul per 1 x 10^6 cells.
IP: 6ug/mg lysate
IHC: 1:1000-1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.25 ul per 1 x 10^6 cells.
Predicted Reactivity
Rat, Dog, Rabbit, Orangutan
Immunogen
Between 187 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse ICLN by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ICLN antibody AAA23778 (lot AAA23778-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse ICLN by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ICLN antibody AAA23778 (lot AAA23778-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human ICLN by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ICLN antibody AAA23778 (lot AAA23778-1) used for IP at 6 ug per reaction. ICLN was also immunoprecipitated by rabbit anti-ICLN antibody BL17109. For blotting immunoprecipitated ICLN, AAA23778 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human ICLN by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ICLN antibody AAA23778 (lot AAA23778-1) used for IP at 6 ug per reaction. ICLN was also immunoprecipitated by rabbit anti-ICLN antibody BL17109. For blotting immunoprecipitated ICLN, AAA23778 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human ICLN by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ICLN (Cat. No. AAA23778 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human ICLN by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ICLN (Cat. No. AAA23778 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of mouse ICLN by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-ICLN (Cat. No. AAA23778 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of mouse ICLN by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-ICLN (Cat. No. AAA23778 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
FCM (Flow Cytometry)
(Detection of human ICLN (shaded) in A549 cells by flow cytometry. Antibody: Rabbit anti-ICLN polyclonal antibody (AAA23778) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human ICLN (shaded) in A549 cells by flow cytometry. Antibody: Rabbit anti-ICLN polyclonal antibody (AAA23778) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of mouse ICLN (shaded) in A20 cells by flow cytometry. Antibody: Rabbit anti-ICLN polyclonal antibody (AAA23778) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of mouse ICLN (shaded) in A20 cells by flow cytometry. Antibody: Rabbit anti-ICLN polyclonal antibody (AAA23778) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Related Product Information for anti-ICLN antibody
Chloride channel nucleotide sensitive 1A (ICLN) is a protein that functions in multiple regulatory pathways. ICLN complexes with numerous cytosolic proteins and performs diverse functions including regulation of small nuclear ribonucleoprotein biosynthesis, platelet activation and cytoskeletal organization.ICLN is also found associated with the plasma membrane where it functions as a chloride current regulator [taken from NCBI Entrez Gene (Gene ID: 1207)].
Product Categories/Family for anti-ICLN antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,215 Da
NCBI Official Full Name
methylosome subunit pICln
NCBI Official Synonym Full Names
chloride channel, nucleotide-sensitive, 1A
NCBI Official Symbol
CLNS1A
NCBI Official Synonym Symbols
CLCI; ICln; CLNS1B
NCBI Protein Information
methylosome subunit pICln; i(Cln); reticulocyte pICln; reticulocyte protein ICln; chloride channel regulatory protein; chloride ion current inducer protein; chloride channel, nucleotide sensitive 1A; chloride conductance regulatory protein ICln
UniProt Protein Name
Methylosome subunit pICln
UniProt Gene Name
CLNS1A
UniProt Synonym Gene Names
CLCI; ICLN; I(Cln); ClCI
UniProt Entry Name
ICLN_HUMAN
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Product Notes
The ICLN clns1a (Catalog #AAA23778) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ICLN Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ICLN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:2000-1:10,000 IP: 6ug/mg lysate IHC: 1:1000-1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.25 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the ICLN clns1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ICLN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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