Human Nucleophosmin (NPM) ELISA Kit | NPM elisa kit
Human Nucleophosmin (NPM) ELISA Kit
Gene Names
NPM1; B23; NPM
Reactivity
Human
Synonyms
Nucleophosmin (NPM); N/A; Human Nucleophosmin (NPM) ELISA Kit; B23; NPM1; Nucleolar Phosphoprotein B23; Numatrin; Nucleophosmin/Nucleoplasmin Family,Member 1; Nucleolar protein NO38; NPM elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of nucleophosmin. No significant cross-reactivity or interference between nucleophosmin and analogues was observed.
Sequence Length
259
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.115ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level nucleophosmin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level nucleophosmin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Related Product Information for NPM elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of nucleophosmin in human tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to nucleophosmin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to nucleophosmin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain nucleophosmin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of nucleophosmin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to nucleophosmin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to nucleophosmin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain nucleophosmin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of nucleophosmin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for NPM elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
28,400 Da
NCBI Official Full Name
nucleophosmin
NCBI Official Synonym Full Names
nucleophosmin (nucleolar phosphoprotein B23, numatrin)
NCBI Official Symbol
NPM1
NCBI Official Synonym Symbols
B23; NPM
NCBI Protein Information
nucleophosmin
UniProt Protein Name
Nucleophosmin
UniProt Gene Name
NPM1
UniProt Synonym Gene Names
NPM; NPM
UniProt Entry Name
NPM_HUMAN
