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WB (Western Blot)
(Detection of human CD20 by western blot. Samples: Whole cell lysate (25 ug) from Ramos and MCF-7 cells prepared using NETN lysis buffer. Antibody: Mouse anti-CD20 monoclonal antibody [L26] (AAA23779 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
Mouse anti-Human CD20 Monoclonal Antibody | anti-CD20 antibody
Mouse anti-CD20 Monoclonal Antibody, Purified [L26]
Gene Names
MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
Purity
Purified
Synonyms
CD20; Monoclonal Antibody; Mouse anti-CD20 Monoclonal Antibody; Purified [L26]; Membrane-spanning 4-domains subfamily A member 1; S7; membrane-spanning 4-domains; subfamily A; member 1; leukocyte surface antigen Leu-16; LEU-16; CVID5; CD20 receptor; CD antigen CD20; Bp35; B-lymphocyte surface antigen B1; B1; B-lymphocyte cell-surface antigen B1; B-lymphocyte antigen CD20; CD20 antigen; MS4A2; membrane spanning 4-domains A1; anti-CD20 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
L26
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide
Concentration
300ug/ml (varies by lot)
Applicable Applications for anti-CD20 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS), Multiplex immunofluorescence (mIF)
Application Notes
WB: 1:1000
IHC: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
IHC-IF: 1:100-1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.25 ul per 1 x 10^6 cells.
mIF: 1:250
IHC: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
IHC-IF: 1:100-1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.25 ul per 1 x 10^6 cells.
mIF: 1:250
Immunogen
Human tonsil B cells
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CD20 by western blot. Samples: Whole cell lysate (25 ug) from Ramos and MCF-7 cells prepared using NETN lysis buffer. Antibody: Mouse anti-CD20 monoclonal antibody [L26] (AAA23779 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human CD20 by western blot. Samples: Whole cell lysate (25 ug) from Ramos and MCF-7 cells prepared using NETN lysis buffer. Antibody: Mouse anti-CD20 monoclonal antibody [L26] (AAA23779 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IHC (Immunohistochemistry)
(Detection of human CD20 (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse anti-CD20 monoclonal antibody [L26] (AAA23779 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry)
(Detection of human CD20 (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse anti-CD20 monoclonal antibody [L26] (AAA23779 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry)
(Detection of human CD20 by immunhistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-CD20 antibody [L26] (AAA23779 lot 1) used at 1:250. Secondary: DyLight 594-conjugated goat anti-mouse IgG .)
IHC (Immunohistochemistry)
(Detection of human CD20 by immunhistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-CD20 antibody [L26] (AAA23779 lot 1) used at 1:250. Secondary: DyLight 594-conjugated goat anti-mouse IgG .)
IHC (Immunohistochemistry)
(Detection of human CD20 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Purified mouse monoclonal anti-CD20 (Clone L26) antibody (AAA23779 Lot2) used at a dilution of 1:500. Detection: DAB. Counterstain: IHC Hematoxylin (blue).)
IHC (Immunohistochemistry)
(Detection of human CD20 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Purified mouse monoclonal anti-CD20 (Clone L26) antibody (AAA23779 Lot2) used at a dilution of 1:500. Detection: DAB. Counterstain: IHC Hematoxylin (blue).)
ICC (Immunocytochemistry)
(Detection of human CD20 by immunocytochemistry. Sample: FFPE section of VLN3G2 cells. Antibody: Purified mouse monoclonal anti-CD20 (Clone L26) antibody (AAA23779 Lot2) used at a dilution of 1:500. Detection: DAB. Counterstain: IHC Hematoxylin (blue).)
ICC (Immunocytochemistry)
(Detection of human CD20 by immunocytochemistry. Sample: FFPE section of VLN3G2 cells. Antibody: Purified mouse monoclonal anti-CD20 (Clone L26) antibody (AAA23779 Lot2) used at a dilution of 1:500. Detection: DAB. Counterstain: IHC Hematoxylin (blue).)
FCM (Flow Cytometry)
(Detection of human CD20 (shaded) in Raji cells by flow cytometry. Antibody: Mouse anti-CD20 monoclonal antibody [L26] (AAA23779) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-mouse IgG .)
FCM (Flow Cytometry)
(Detection of human CD20 (shaded) in Raji cells by flow cytometry. Antibody: Mouse anti-CD20 monoclonal antibody [L26] (AAA23779) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-mouse IgG .)
Related Product Information for anti-CD20 antibody
CD20 is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. CD20 is a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells [taken from NCBI Entrez Gene (Gene ID: 931)].
Product Categories/Family for anti-CD20 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33077 MW
NCBI Official Full Name
B-lymphocyte antigen CD20
NCBI Official Synonym Full Names
membrane spanning 4-domains A1
NCBI Official Symbol
MS4A1
NCBI Official Synonym Symbols
B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
NCBI Protein Information
B-lymphocyte antigen CD20
UniProt Protein Name
B-lymphocyte antigen CD20
UniProt Gene Name
MS4A1
UniProt Synonym Gene Names
CD20
UniProt Entry Name
CD20_HUMAN
Similar Products
Product Notes
The CD20 ms4a1 (Catalog #AAA23779) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti-CD20 Monoclonal Antibody, Purified [L26] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD20 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS), Multiplex immunofluorescence (mIF). WB: 1:1000 IHC: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IHC-IF: 1:100-1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.25 ul per 1 x 10^6 cells. mIF: 1:250. Researchers should empirically determine the suitability of the CD20 ms4a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD20, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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