Mouse anti-Human, Mouse Sodium Potassium ATPase alpha-1 Monoclonal Antibody | anti-ATP1A1 antibody

Mouse anti-Sodium Potassium ATPase alpha-1 Monoclonal Antibody [464.6]

Cat. Num. AAA23784

• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
• Purity: Purified
• Synonyms: Sodium Potassium ATPase alpha-1; Monoclonal Antibody; Mouse anti-Sodium Potassium ATPase alpha-1 Monoclonal Antibody [464.6]; Na; K-ATPase catalytic subunit alpha-A protein; sodium-potassium-ATPase; alpha 1 polypeptide; sodium-potassium ATPase catalytic subunit alpha-1; sodium/potassium-transporting ATPase subunit alpha-1; Na+/K+ ATPase 1; K-ATPase alpha-1 subunit; K-ATPase; alpha-A catalytic polypeptide; Na(+)/K(+) ATPase alpha-1 subunit; CMT2DD; ATPase; Na+/K+ transporting; sodium pump subunit alpha-1; Sodium/potassium-transporting ATPase subunit alpha-1; ATPase Na+/K+ transporting subunit alpha 1; anti-ATP1A1 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: 464.6
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
• Concentration: 1000ug/ml (varies by lot)

• Applicable Applications for anti-ATP1A1 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: WB: 1:1000; IP: 6 ul/mg lysate; IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Immunogen: Full length protein
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse Sodium Potassium ATPase alpha-1 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, BW5147.3, TCMK-1, and CH27 cells prepared using NETN lysis buffer. Antibody: Mouse anti-Sodium Potassium ATPase alpha-1-Recombinant monoclonal antibody [BL-464.6] (AAA23784 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

WB (Western Blot)

(Detection of human Sodium Potassium ATPase alpha-1 by western blot. Samples: Whole cell lysate (10 ug) from MDA-MB-435, LNCaP, UACC-62, HEK293T, and HeLa cells prepared using NETN lysis buffer. Antibody: Mouse anti-Sodium Potassium ATPase alpha-1 monoclonal antibody [BL-464.6] (AAA23784 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

IP (Immunoprecipitation)

(Detection of human Sodium Potassium ATPase alpha-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibody: Mouse anti-Sodium Potassium ATPase alpha-1-Recombinant monoclonal antibody [BL-464.6] (AAA23784 lot 1) used for IP at 20 ul/mg lysate. For blotting immunoprecipitated Sodium Potassium ATPase alpha-1, A500-031 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

IHC (Immunohistochemistry)

(Detection of human Sodium Potassium ATPase alpha-1 by immunohistochemistry. Sample: FFPE section of colon carcinoma. Antibody: Mouse anti-Sodium Potassium ATPase alpha-1 monoclonal antibody [464.6] (AAA23784 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG .)

IHC (Immunohistochemistry)

(Detection of mouse Sodium Potassium ATPase alpha-1 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Mouse anti-Sodium Potassium ATPase alpha-1 monoclonal antibody [464.6] (AAA23784 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG .)

ICC (Immunocytochemistry)

(Detection of human Sodium Potassium ATPase alpha-1 by immunocytochemistry. Sample: FFPE section of MDA-MB-435 cells.Antibody: Mouse anti-Sodium Potassium ATPase alpha-1 monoclonal antibody [464.6] (AAA23784 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG .)

Related Product Information for anti-ATP1A1 antibody

Na+/K+-ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). [taken from NCBI Entrez Gene (Gene ID: 476)].

Product Categories/Family for anti-ATP1A1 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 21361181
• NCBI GeneID: 476
• NCBI Accession #: NP_000692.2
• NCBI GenBank Nucleotide #: NM_000701.7
• UniProt Accession #: P05023; Q16689; Q6LDM4; Q9UCN1; Q9UJ20; Q9UJ21; B2RBR6; B7Z3U6
• Molecular Weight: 112,896 Da
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit alpha-1 isoform a
• NCBI Official Synonym Full Names: ATPase, Na+/K+ transporting, alpha 1 polypeptide
• NCBI Official Symbol: ATP1A1
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit alpha-1; sodium pump 1; Na+/K+ ATPase 1; Na,K-ATPase alpha-1 subunit; sodium pump subunit alpha-1; Na+, K+ ATPase alpha subunit; Na(+)/K(+) ATPase alpha-1 subunit; Na, K-ATPase, alpha-A catalytic polypeptide; s
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit alpha-1
• UniProt Gene Name: ATP1A1
• UniProt Synonym Gene Names: Na(+)/K(+) ATPase alpha-1 subunit
• UniProt Entry Name: AT1A1_HUMAN