WB (Western Blot)
(Detection of mouse KAP-1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (AAA23792 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-Cytoskeletal Actin recombinant monoclonal antibody .)
KAP-1 recombinant antibody
Rabbit anti-KAP-1 Recombinant Monoclonal Antibody [BL-248-2G6]
IP: 20 ul/mg lysate
IHC: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
ICC: 1:100-1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse KAP-1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (AAA23792 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-Cytoskeletal Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of mouse KAP-1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (AAA23792 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-Cytoskeletal Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human KAP-1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, U2OS, HEK293T, Hep-G2, K-562, RKO, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (AAA23792 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-Cytoskeletal Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human KAP-1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, U2OS, HEK293T, Hep-G2, K-562, RKO, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (AAA23792 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-Cytoskeletal Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation)
(Detection of human KAP-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (AAA23792 lot 2) used for IP at 20 ul per reaction. KAP-1 was also immunoprecipitated by a previous lot of this antibody (lot AAA23792-1) and rabbit anti-KAP-1 antibodies . For blotting immunoprecipitated KAP-1, AAA23792 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human KAP-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (AAA23792 lot 2) used for IP at 20 ul per reaction. KAP-1 was also immunoprecipitated by a previous lot of this antibody (lot AAA23792-1) and rabbit anti-KAP-1 antibodies . For blotting immunoprecipitated KAP-1, AAA23792 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)
IHC (Immunohistochemistry)
(Detection of mouse KAP-1 in FFPE colon carcinoma CT26 by IHC. Antibody: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (AAA23792 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of mouse KAP-1 in FFPE colon carcinoma CT26 by IHC. Antibody: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (AAA23792 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human KAP-1 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (AAA23792 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human KAP-1 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (AAA23792 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human KAP-1 in FFPE SK-MEL-28 cells by ICC. Antibody: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (AAA23792 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human KAP-1 in FFPE SK-MEL-28 cells by ICC. Antibody: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (AAA23792 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry)
(Detection of human KAP-1 by flow cytometry. Sample: Jurkat cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody: 1E6 cells were incubated with 1 ul of rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (AAA23792) (red shaded) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human KAP-1 by flow cytometry. Sample: Jurkat cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody: 1E6 cells were incubated with 1 ul of rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (AAA23792) (red shaded) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
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Product Notes
The KAP-1 trim28 (Catalog #AAA23792) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-KAP-1 Recombinant Monoclonal Antibody [BL-248-2G6] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KAP-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 20 ul/mg lysate IHC: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ICC: 1:100-1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the KAP-1 trim28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KAP-1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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