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WB (Western Blot)
(Detection of human and mouse MED12 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, A-549, MCF-7, Hep-G2, K-562, RKO, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MED12 recombinant monoclonal antibody (AAA23825 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-eEF2 .)
MED12 recombinant antibody
Rabbit anti-MED12 Recombinant Monoclonal Antibody [BLR084G]
Gene Names
MED12; OKS; FGS1; HOPA; OPA1; OHDOX; ARC240; CAGH45; MED12S; TNRC11; TRAP230
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
MED12; Monoclonal Recombinant Antibody; Rabbit anti-MED12 Recombinant Monoclonal Antibody [BLR084G]; OHDOX; trinucleotide repeat-containing gene 11 protein; trinucleotide repeat containing 11 (THR-associated protein; 230 kDa subunit); TRAP230; TNRC11; thyroid hormone receptor-associated protein; 230 kDa subunit; thyroid hormone receptor-associated protein complex 230 kDa component; putative mediator subunit 12; OPA-containing protein; OKS; FGS1; activator-recruited cofactor 240 kDa component; ARC240; OPA1; CAGH45; mediator of RNA polymerase II transcription; subunit 12 homolog; HOPA; human opposite paired; MED12S; Mediator complex subunit 12; mediator of RNA polymerase II transcription subunit 12; CAG repeat protein 45; Mediator of RNA polymerase II transcription subunit 12; mediator complex subunit 12; MED12 recombinant antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR084G
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
250ug/ml (varies by lot)
Applicable Applications for MED12 recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000 (3-8% Tris-acetate gel recommended)
IP: 20 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
IP: 20 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
Immunogen
Between 2127 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse MED12 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, A-549, MCF-7, Hep-G2, K-562, RKO, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MED12 recombinant monoclonal antibody (AAA23825 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-eEF2 .)
WB (Western Blot)
(Detection of human and mouse MED12 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, A-549, MCF-7, Hep-G2, K-562, RKO, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MED12 recombinant monoclonal antibody (AAA23825 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-eEF2 .)
IP (Immunoprecipitation)
(Detection of human MED12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MED12 recombinant monoclonal antibody (AAA23825 lot 1) used for IP at 20 ul/mg lysate. MED12 was also immunoprecipitated by rabbit anti-MED12 antibody For blotting immunoprecipitated MED12, AAA23825 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human MED12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MED12 recombinant monoclonal antibody (AAA23825 lot 1) used for IP at 20 ul/mg lysate. MED12 was also immunoprecipitated by rabbit anti-MED12 antibody For blotting immunoprecipitated MED12, AAA23825 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of mouse MED12 in FFPE renal carcinoma by IHC. Antibody: Rabbit anti-MED12 recombinant monoclonal antibody (AAA23825 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of mouse MED12 in FFPE renal carcinoma by IHC. Antibody: Rabbit anti-MED12 recombinant monoclonal antibody (AAA23825 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human MED12 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-MED12 recombinant monoclonal antibody (AAA23825 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human MED12 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-MED12 recombinant monoclonal antibody (AAA23825 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human MED12 in FFPE HCT-116 cells by ICC. Antibody: Rabbit anti-MED12 recombinant monoclonal antibody (AAA23825 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human MED12 in FFPE HCT-116 cells by ICC. Antibody: Rabbit anti-MED12 recombinant monoclonal antibody (AAA23825 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry)
(Detection of human MED12 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-MED12 recombinant monoclonal antibody (AAA23825) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human MED12 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-MED12 recombinant monoclonal antibody (AAA23825) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for MED12 recombinant antibody
MED12 is a component of the multisubunit mediator complex that functions as an interface between gene-specific transcription factors and the RNA Pol II/general transcription factor machinery. The mediator complex was first identified as a coactivator of the thyroid hormone receptor and has since been observed to function as a coactivator of a variety of transcription factors. In this context, MED12 interaction with beta-catenin has been shown to be required for Wnt-signaling dependent transcription.
Product Categories/Family for MED12 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
243,081 Da
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 12
NCBI Official Synonym Full Names
mediator complex subunit 12
NCBI Official Symbol
MED12
NCBI Official Synonym Symbols
OKS; FGS1; HOPA; OPA1; OHDOX; ARC240; CAGH45; MED12S; TNRC11; TRAP230
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 12; CAG repeat protein 45; human opposite paired; OPA-containing protein; putative mediator subunit 12; activator-recruited cofactor 240 kDa component; trinucleotide repeat-containing gene 11 protein; th
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 12
UniProt Gene Name
MED12
UniProt Synonym Gene Names
ARC240; CAGH45; HOPA; KIAA0192; TNRC11; TRAP230; ARC240; Trap230
UniProt Entry Name
MED12_HUMAN
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Product Notes
The MED12 med12 (Catalog #AAA23825) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MED12 Recombinant Monoclonal Antibody [BLR084G] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MED12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:1000 (3-8% Tris-acetate gel recommended) IP: 20 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the MED12 med12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MED12, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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