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WB (Western Blot)
(Detection of human ThPOK by western blot. Samples: Whole cell lysate (50 ug) from LNCaP, MCF-7, HEK293T, HeLa, and OVCAR-3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ThPOK recombinant monoclonal antibody (AAA23860 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
ThPOK recombinant antibody
Rabbit anti-ThPOK Recombinant Monoclonal Antibody [BLR191J]
Gene Names
ZBTB7B; CKROX; THPOK; ZFP67; ZBTB15; ZFP-67; c-KROX; hcKROX; ZNF857B
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
ThPOK; Monoclonal Recombinant Antibody; Rabbit anti-ThPOK Recombinant Monoclonal Antibody [BLR191J]; zinc finger and BTB domain containing 15; ZNF857B; zinc finger protein Th-POK; zinc finger protein 857B; c-KROX; zinc finger protein 67 homolog; zinc finger and BTB domain-containing protein 7B; Zinc finger and BTB domain-containing protein 15; ZFP67; ZBTB15; THPOK; T-helper-inducing POZ/Krueppel-like factor; hcKROX; krueppel-related zinc finger protein cKrox; CKROX; ZFP-67; Zinc finger and BTB domain-containing protein 7B; zinc finger and BTB domain containing 7B; ThPOK recombinant antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR191J
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
250ug/ml (varies by lot)
Applicable Applications for ThPOK recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IP: 20 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells.
IP: 20 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells.
Immunogen
Between 250 and 300
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human ThPOK by western blot. Samples: Whole cell lysate (50 ug) from LNCaP, MCF-7, HEK293T, HeLa, and OVCAR-3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ThPOK recombinant monoclonal antibody (AAA23860 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human ThPOK by western blot. Samples: Whole cell lysate (50 ug) from LNCaP, MCF-7, HEK293T, HeLa, and OVCAR-3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ThPOK recombinant monoclonal antibody (AAA23860 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation)
(Detection of human ThPOK by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from LNCaP cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-ThPOK recombinant monoclonal antibody (AAA23860 lot 1) used for IP at 20 ul/mg lysate. ThPOK was also immunoprecipitated by a second antibody against a different epitope of ThPOK . For blotting immunoprecipitated ThPOK, AAA23860 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human ThPOK by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from LNCaP cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-ThPOK recombinant monoclonal antibody (AAA23860 lot 1) used for IP at 20 ul/mg lysate. ThPOK was also immunoprecipitated by a second antibody against a different epitope of ThPOK . For blotting immunoprecipitated ThPOK, AAA23860 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of mouse ThPOK by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-ThPOK recombinant monoclonal antibody (AAA23860 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry)
(Detection of mouse ThPOK by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-ThPOK recombinant monoclonal antibody (AAA23860 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry)
(Detection of human ThPOK by immunohistochemistry. Sample: FFPE section of prostate carcinoma. Antibody: Rabbit anti-ThPOK recombinant monoclonal antibody (AAA23860 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry)
(Detection of human ThPOK by immunohistochemistry. Sample: FFPE section of prostate carcinoma. Antibody: Rabbit anti-ThPOK recombinant monoclonal antibody (AAA23860 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of mouse ThPOK by immunocytochemistry. Sample: FFPE section of RenCa cells. Antibody: Rabbit anti-ThPOK recombinant monoclonal antibody (AAA23860). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of mouse ThPOK by immunocytochemistry. Sample: FFPE section of RenCa cells. Antibody: Rabbit anti-ThPOK recombinant monoclonal antibody (AAA23860). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human ThPOK by immunocytochemistry. Sample: FFPE section of Ovcar-3 cells. Antibody: Rabbit anti-ThPOK recombinant monoclonal antibody (AAA23860 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human ThPOK by immunocytochemistry. Sample: FFPE section of Ovcar-3 cells. Antibody: Rabbit anti-ThPOK recombinant monoclonal antibody (AAA23860 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human ThPOK (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-ThPOK recombinant monoclonal antibody (AAA23860) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human ThPOK (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-ThPOK recombinant monoclonal antibody (AAA23860) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Related Product Information for ThPOK recombinant antibody
ThPOK is a transcription factor that acts as a key regulator of lineage commitment of immature T-cell precursors. ThPOK is necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O15156].
Product Categories/Family for ThPOK recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,584 Da
NCBI Official Synonym Full Names
zinc finger and BTB domain containing 7B
NCBI Official Symbol
ZBTB7B
NCBI Official Synonym Symbols
CKROX; THPOK; ZFP67; ZBTB15; ZFP-67; c-KROX; hcKROX; ZNF857B
NCBI Protein Information
zinc finger and BTB domain-containing protein 7B
UniProt Protein Name
Zinc finger and BTB domain-containing protein 7B
UniProt Gene Name
ZBTB7B
UniProt Synonym Gene Names
ZBTB15; ZFP67; ZNF857B; hcKrox; Zfp-67
UniProt Entry Name
ZBT7B_HUMAN
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Product Notes
The ThPOK zbtb7b (Catalog #AAA23860) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ThPOK Recombinant Monoclonal Antibody [BLR191J] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ThPOK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 20 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the ThPOK zbtb7b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ThPOK, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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