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Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Calretinin Mouse Monoclonal Antibody (CALB2/2602). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mouse anti-Human Calretinin/Calbindin 2 Monoclonal Antibody | anti-CALB2 antibody
Calretinin/Calbindin 2 (Mesothelioma Marker)
Gene Names
CALB2; CR; CAL2; CAB29
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA at 1.0mg/ml
Synonyms
Calretinin/Calbindin 2, Antibody; Calretinin/Calbindin 2 (Mesothelioma Marker); 29kDa calbindin; CAB29; CAL2; CALB2; Calbindin 2; Calbindin D29K; Calretinin; CR; anti-CALB2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
CALB2/2602
Specificity
It recognizes a protein of about 29kDa, which is identified as Calretinin (also known as Calbindin 2). Calretinin is a vitamin D-dependent calcium-binding protein involved in calcium signaling. It is present in subsets of neurons throughout the brain and spinal chord, including sensory ganglia. Antibody to calretinin is useful in differentiating mesothelioma from adenocarcinomas of the lung. It also aids in differentiating adrenal cortical neoplasms from pheochromocytomas.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Sequence Length
87
Applicable Applications for anti-CALB2 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes
WB: 0.5-2ug/ml
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Positive Control
Brain, Testis or Mesothelioma.
Immunogen
Recombinant human Calretinin (Calbindin 2) protein fragment (around aa23-242) (exact sequence is proprietary)
Cellular Localization
Cytoplasmic and Nuclear
Hu-chromosome Location
16q22.2
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Calretinin Mouse Monoclonal Antibody (CALB2/2602). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Calretinin Mouse Monoclonal Antibody (CALB2/2602). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS-PAGE
(SDS-PAGE Analysis Purified Calretinin Mouse Monoclonal Antibody (CALB2/2602). Confirmation of Purity and Integrity of Antibody)
SDS-PAGE
(SDS-PAGE Analysis Purified Calretinin Mouse Monoclonal Antibody (CALB2/2602). Confirmation of Purity and Integrity of Antibody)
WB (Western Blot)
(Western Blot Analysis of human Brain tissue lysate using Calretinin Mouse Monoclonal Antibody (CALB2/2602).)
WB (Western Blot)
(Western Blot Analysis of human Brain tissue lysate using Calretinin Mouse Monoclonal Antibody (CALB2/2602).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Mesothelioma stained with Calretinin Mouse Monoclonal Antibody (CALB2/2602).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Mesothelioma stained with Calretinin Mouse Monoclonal Antibody (CALB2/2602).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Calretinin Mouse Monoclonal Antibody (CALB2/2602).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Calretinin Mouse Monoclonal Antibody (CALB2/2602).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Cerebellum stained with Calretinin Mouse Monoclonal Antibody (CALB2/2602).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Cerebellum stained with Calretinin Mouse Monoclonal Antibody (CALB2/2602).)
References
1. Doglioni, C., et al. 1996. Am. J. Surg. Pathol. 20: 1037-1046. 2. Rogers, J.H. 1987. J. Cell Biol. 105: 1343-1353.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
29kDa
NCBI Official Full Name
calretinin, partial
NCBI Official Synonym Full Names
calbindin 2
NCBI Official Symbol
CALB2
NCBI Official Synonym Symbols
CR; CAL2; CAB29
NCBI Protein Information
calretinin
UniProt Protein Name
Calretinin
UniProt Gene Name
CALB2
UniProt Synonym Gene Names
CAB29; CR
UniProt Entry Name
CALB2_HUMAN
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Product Notes
The CALB2 calb2 (Catalog #AAA23938) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Calretinin/Calbindin 2 (Mesothelioma Marker) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Calretinin/Calbindin 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. WB: 0.5-2ug/ml Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the CALB2 calb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calretinin/Calbindin 2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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