Rabbit anti-Human DLL3 Polyclonal Antibody | anti-DLL3 antibody

DLL3, CT (DLL3, Delta-like protein 3, Drosophila Delta homolog 3) (APC)

Cat. Num. AAA26850

• Gene Names: DLL3; SCDO1
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DLL3; Polyclonal Antibody; CT (DLL3; Delta-like protein 3; Drosophila Delta homolog 3) (APC); Drosophila Delta homolog 3; anti-DLL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DLL3. Species Crossreactivity: mouse and rat
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Concentration: 0.5 mg/ml (varies by lot)

• Applicable Applications for anti-DLL3 antibody: Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB); ; Other applications not tested.
• Application Notes: IHC: Formalin fixed, paraffin embedded tissues. Heat induced antigen retrieval with EDTA Buffer, pH 9.0 is recommended.; ; Optimal dilutions to be determined by the researcher.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 519-548 in the C-terminal region of human DLL3
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

IF (Immunofluorescence)

(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling DLL3 with (1:25), followed by Dylight 488- conjugated goat anti- rabbit IgG secondary antibody at (1:200) (green). Immunofluorescence image showing nucleus and weak cytoplasm staining on U-2 OScell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (1:100) (red).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin embedded human testis tissue using performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at Rt, antigen retrieval was by heat mediation with a EDTA buffer, pH 9.0. Samples were incubated (1:500) for 1 hours at RT. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of parffin-embedded human brain tissue using performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at RT, antigen retrievalwas by heat heat mediation with a EDTA buffer, pH 9.0. Samples were incubated with (1:500) for 1 hours at RT. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)

Application Data

(All lanes: (1:1000) Lane 1: NCI-H1299 whole cell lysateLane 2: Human brain tissue lysateLane 3: Mouse brain tissue lysateLane 4: Rat brain whole cell lysate Lysates/proteins at 20/lane. Secondary IgG (H+L) (HRP) goat anti-rabbit (1:10000) Predicted band size: 65 KD Blocking/Dilution buffer: 5% NFDM/TBST.)

Application Data

(All lanes: (1:1000) Lane 1: A375 whole cell lysateLane 2: U-251 MG whole cell lysateLane 3: Human brain lysate Lane 5: Mouse brain lysate Lane 6: Rat brain lysate Lysates/proteins at 20/lane. Secondary IgG (H+L) (HRP) goat anti-rabbit (1:10000) Predicted band size: 65 KD Blocking Dilution buffer: 5% NFDM/TBST.)

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of DLL3 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohistochemistry)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with DLL3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of DLL3 Antibody (C-term) in A375 cell line lysates (35ug/lane). DLL3 (arrow) was detected using the purified Pab.)

Related Product Information for anti-DLL3 antibody

DLL3 encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain.

Product Categories/Family for anti-DLL3 antibody

Antibodies; Notch Proteins

References

Yerges, L.M. J. Bone Miner. Res. 24 (12), 2039-2049 (2009). Heuss, S.F. Proc. Natl. Acad. Sci. U.S.A. 105 (32), 11212-11217 (2008). Maisenbacher, M.K. Hum. Genet. 116 (5), 416-419 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 45243561
• NCBI GeneID: 10683
• NCBI Accession #: NP_982353.1
• NCBI GenBank Nucleotide #: NM_203486.2
• UniProt Accession #: Q9NYJ7; Q8NBS4; E9PFG2
• NCBI Official Full Name: delta-like protein 3 isoform 2
• NCBI Official Synonym Full Names: delta like canonical Notch ligand 3
• NCBI Official Symbol: DLL3
• NCBI Official Synonym Symbols: SCDO1
• NCBI Protein Information: delta-like protein 3
• UniProt Protein Name: Delta-like protein 3
• UniProt Gene Name: DLL3
• UniProt Synonym Gene Names: Delta3