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IHC (Immunohistchemistry)
(Immunohistochemical analysis using PD-L1 Mouse mAb PD-L1 IHC Controls (paraffin-embedded cell pellets, HDLM-2 (left) or PC-3 (right).)
Mouse anti-Human PDL1, CT (Programmed Cell Death 1 Ligand 1, B7-H1, CD274) Monoclonal Antibody | anti-PDL1 antibody
PDL1, CT (Programmed Cell Death 1 Ligand 1, B7-H1, CD274)
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Supernatant
Synonyms
PDL1; CT (Programmed Cell Death 1 Ligand 1; B7-H1; CD274); Monoclonal Antibody; CT; anti-PDL1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
405.9A11
Specificity
Recognizes endogenous levels of human PD-L1.
Purity/Purification
Supernatant
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 0.1mg/ml BSA, 150mM sodium chloride, <0.02% sodium azide, 50% glycerol.
Applicable Applications for anti-PDL1 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunohistochemistry (paraffin): 1:200
Immunohistochemistry (paraffin): 1:200
Immunogen
Synthetic peptide corresponding to human PD-L1 at C-terminal.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
IHC (Immunohistchemistry)
(Immunohistochemical analysis using PD-L1 Mouse mAb PD-L1 IHC Controls (paraffin-embedded cell pellets, HDLM-2 (left) or PC-3 (right).)
IHC (Immunohistchemistry)
(Immunohistochemical analysis using PD-L1 Mouse mAb PD-L1 IHC Controls (paraffin-embedded cell pellets, HDLM-2 (left) or PC-3 (right).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of parffin-embedded human placenta using PD-L1 Mouse mAb.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of parffin-embedded human placenta using PD-L1 Mouse mAb.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of parffin-embedded human tonsil using PD-L1 Mouse mAb.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of parffin-embedded human tonsil using PD-L1 Mouse mAb.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PD-L1 Mouse mAb.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PD-L1 Mouse mAb.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PD-L1 Mouse mAb.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PD-L1 Mouse mAb.)
WB (Western Blot)
(Western blot analysis of extracts from various cell lines using PD-L1 Mouse mAb (upper) or B-Actin Rabbit mAb (lower).)
WB (Western Blot)
(Western blot analysis of extracts from various cell lines using PD-L1 Mouse mAb (upper) or B-Actin Rabbit mAb (lower).)
Related Product Information for anti-PDL1 antibody
Programmed cell death 1 ligand 1 (PD-L1, B7-H1, CD274) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. The PD-L1 ligand binds the PD-1 transmembrane receptor and inhibits T cell activation. PD-L1 was discovered following a search for novel B7 protein homologs and was later shown to be expressed by antigen presenting cells, activated T cells, and tissues including placenta, heart, and lung (1-3). Similar in structure to related B7 family members, PD-L1 protein contains extracellular IgV and IgC domains and a short, cytoplasmic region. Research studies demonstrate that PD-L1 is expressed in several tumor types, including melanoma, ovary, colon, lung, breast, and renal cell carcinomas (4-6). Expression of PD-L1 in cancer is associated with tumor infiltrating lymphocytes, which mediate PD-L1 expression through the release of interferon gamma (7). Additional research links PD-L1 expression to cancers associated with viral infections (8,9).
Product Categories/Family for anti-PDL1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,275 Da
NCBI Official Full Name
programmed cell death 1 ligand 1 isoform b
NCBI Official Synonym Full Names
CD274 molecule
NCBI Official Symbol
CD274
NCBI Official Synonym Symbols
B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
NCBI Protein Information
programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1
UniProt Protein Name
Programmed cell death 1 ligand 1
UniProt Gene Name
CD274
UniProt Synonym Gene Names
B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
UniProt Entry Name
PD1L1_HUMAN
Similar Products
Product Notes
The PDL1 cd274 (Catalog #AAA26907) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PDL1, CT (Programmed Cell Death 1 Ligand 1, B7-H1, CD274) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PDL1, CT (Programmed Cell Death 1 Ligand 1, B7-H1, CD274) can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Immunohistochemistry (paraffin): 1:200. Researchers should empirically determine the suitability of the PDL1 cd274 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDL1, CT (Programmed Cell Death 1 Ligand 1, B7-H1, CD274), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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