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WB (Western Blot)
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WB (Western Blot) (Detection of human MED1 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, and SW620 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807 Lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Cytoskeletal Actin recombinant monoclonal .)

MED1 recombinant antibody

Rabbit anti-MED1 Recombinant Monoclonal Antibody [BLR037F]

Gene Names
MED1; PBP; CRSP1; RB18A; TRIP2; PPARBP; CRSP200; DRIP205; DRIP230; PPARGBP; TRAP220
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
MED1; Monoclonal Recombinant Antibody; Rabbit anti-MED1 Recombinant Monoclonal Antibody [BLR037F]; PPAR-binding protein; PPARBP; PPARGBP; RB18A; Thyroid receptor-interacting protein 2; TR-interacting protein 2; TRIP-2; peroxisome proliferator-activated receptor-binding protein; TRAP220; vitamin D receptor-interacting protein 230 kD; activator-recruited cofactor 205 kDa component; p53 regulatory protein RB18A; mediator of RNA polymerase II transcription subunit 1; Mediator complex subunit 1; DRIP230; DRIP205; CRSP200; CRSP1; ARC205; TRIP2; PBP; Mediator of RNA polymerase II transcription subunit 1; mediator complex subunit 1; MED1 recombinant antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR037F
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
100ug/ml (varies by lot)
Applicable Applications for MED1 recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IP: 20 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
Predicted Reactivity
Orangutan
Immunogen
Between 1531 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human MED1 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, and SW620 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807 Lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Cytoskeletal Actin recombinant monoclonal .)

WB (Western Blot) (Detection of human MED1 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, and SW620 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807 Lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Cytoskeletal Actin recombinant monoclonal .)

WB (Western Blot)

(Detection of mouse MED1 by western blot. Samples: Whole cell lysate (10 ug) from EL4, mIMCD-3, RenCa, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807 Lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Cytoskeletal Actin recombinant monoclonal .)

WB (Western Blot) (Detection of mouse MED1 by western blot. Samples: Whole cell lysate (10 ug) from EL4, mIMCD-3, RenCa, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807 Lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Cytoskeletal Actin recombinant monoclonal .)

IP (Immunoprecipitation)

(Detection of human MED1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807 Lot 2) used for IP at 20 ul/mg lysate. MED1 was also immunoprecipitated by a previous lot of this antibody (AAA23807-1) and rabbit anti-MED1 antibody For blotting immunoprecipitated MED1, AAA23807 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)

IP (Immunoprecipitation) (Detection of human MED1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807 Lot 2) used for IP at 20 ul/mg lysate. MED1 was also immunoprecipitated by a previous lot of this antibody (AAA23807-1) and rabbit anti-MED1 antibody For blotting immunoprecipitated MED1, AAA23807 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)

IHC (Immunohistochemistry)

(Detection of human MED1 in FFPE lung carcinoma by immunohistochemistry. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807 Lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry) (Detection of human MED1 in FFPE lung carcinoma by immunohistochemistry. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807 Lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry)

(Detection of mouse MED1 in FFPE renal cell carcinoma by immunohistochemistry. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807 Lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry) (Detection of mouse MED1 in FFPE renal cell carcinoma by immunohistochemistry. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807 Lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human MED1 in FFPE HCT 116 cells by immunocytochemistry. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807 Lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry) (Detection of human MED1 in FFPE HCT 116 cells by immunocytochemistry. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807 Lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry)

(Detection of mouse MED1 (shaded) in mIMCD-3 cells by flow cytometry. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of mouse MED1 (shaded) in mIMCD-3 cells by flow cytometry. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry)

(Detection of human MED1 (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of human MED1 (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody (AAA23807) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Related Product Information for MED1 recombinant antibody
MED1 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q15648].
Product Categories/Family for MED1 recombinant antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
168,478 Da
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 1
NCBI Official Synonym Full Names
mediator complex subunit 1
NCBI Official Symbol
MED1
NCBI Official Synonym Symbols
PBP; CRSP1; RB18A; TRIP2; PPARBP; CRSP200; DRIP205; DRIP230; PPARGBP; TRAP220
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 1; ARC205; TRIP-2; PPAR binding protein; PPAR-binding protein; PPARG binding protein; TR-interacting protein 2; p53 regulatory protein RB18A; thyroid receptor interacting protein 2; thyroid receptor-inte
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 1
UniProt Gene Name
MED1
UniProt Synonym Gene Names
ARC205; CRSP1; CRSP200; DRIP205; DRIP230; PBP; PPARBP; PPARGBP; RB18A; TRAP220; TRIP2; ARC205; PBP; PPAR-binding protein; Trap220; TR-interacting protein 2; TRIP-2
UniProt Entry Name
MED1_HUMAN

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Product Notes

The MED1 med1 (Catalog #AAA23807) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MED1 Recombinant Monoclonal Antibody [BLR037F] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MED1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 20 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the MED1 med1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MED1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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