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IP (Immunoprecipitation)
(Immunoprecipitating GARS in Hela whole cell lysateLane 1: Rabbit monoclonal IgG (1ug)instead of AAA26911 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA26911 (8ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (20ug))
Rabbit anti-Human Annexin A2 Polyclonal Antibody | anti-ANXA2 antibody
Rabbit anti-human Annexin A2 polyclonal Antibody
Gene Names
ANXA2; P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
Annexin A2; Polyclonal Antibody; Rabbit anti-human Annexin A2 polyclonal Antibody; Annexin II; Annexin-2; Calpactin I heavy chain; Calpactin-1 heavy chain; Chromobindin-8; Lipocortin II; Placental anticoagulant protein IV; PAP-IV; ANXA2; ANX2; ANX2L4; CAL1H; LPC2D; anti-ANXA2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Sequence Length
339
Applicable Applications for anti-ANXA2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
Recommended dilution: WB:1:500-2000, IHC:1:20-1:200
Immunogen
Recombinant human Annexin A2 protein (2-339aa)
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-30757 / sc-30759 / sc-1924 / sc-9061
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
IP (Immunoprecipitation)
(Immunoprecipitating GARS in Hela whole cell lysateLane 1: Rabbit monoclonal IgG (1ug)instead of AAA26911 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA26911 (8ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (20ug))
IP (Immunoprecipitation)
(Immunoprecipitating GARS in Hela whole cell lysateLane 1: Rabbit monoclonal IgG (1ug)instead of AAA26911 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA26911 (8ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (20ug))
IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells using AAA26911 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells using AAA26911 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human liver cancer using AAA26911 at dilution of 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human liver cancer using AAA26911 at dilution of 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human placenta using AAA26911 at dilution 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human placenta using AAA26911 at dilution 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human lung tissue using AAA26911 at dilution 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human lung tissue using AAA26911 at dilution 1:100)
WB (Western Blot)
(Western BlotPositive WB detected in:Hela whole cell lysate,K562 whole cell lysate,NIH/3T3 whole cell lysate,A549 whole cell lysate,PC3 whole cell lysate,U251 whole cell lysate,Mouse lung tissue,Mouse liver tissue,Mouse kidney tissueAll lanes: ANXA2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 39,41 kDaObserved band size: 39 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in:Hela whole cell lysate,K562 whole cell lysate,NIH/3T3 whole cell lysate,A549 whole cell lysate,PC3 whole cell lysate,U251 whole cell lysate,Mouse lung tissue,Mouse liver tissue,Mouse kidney tissueAll lanes: ANXA2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 39,41 kDaObserved band size: 39 kDa)
WB (Western Blot)
(Western blotAll lanes: Annexin A2 antibody at 2ug/ml+Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 39,41 kDaObserved band size: 39 kDa)
WB (Western Blot)
(Western blotAll lanes: Annexin A2 antibody at 2ug/ml+Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 39,41 kDaObserved band size: 39 kDa)
Related Product Information for anti-ANXA2 antibody
Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,411 Da
NCBI Official Full Name
annexin A2 isoform 2
NCBI Official Synonym Full Names
annexin A2
NCBI Official Symbol
ANXA2
NCBI Official Synonym Symbols
P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270
NCBI Protein Information
annexin A2
UniProt Protein Name
Annexin A2
UniProt Gene Name
ANXA2
UniProt Synonym Gene Names
ANX2; ANX2L4; CAL1H; LPC2D; PAP-IV
UniProt Entry Name
ANXA2_HUMAN
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Product Notes
The ANXA2 anxa2 (Catalog #AAA26911) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Annexin A2 polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Annexin A2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). Recommended dilution: WB:1:500-2000, IHC:1:20-1:200. Researchers should empirically determine the suitability of the ANXA2 anxa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Annexin A2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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