Rabbit STAT5B Polyclonal Antibody | anti-STAT5B antibody

STAT5B Antibody

Cat. Num. AAA26968

• Gene Names: STAT5B; STAT5
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: STAT5B, Antibody; STAT5B Antibody; Signal transducer and activator of transcription 5B; STAT5B; anti-STAT5B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 787

• Applicable Applications for anti-STAT5B antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1: 500-1: 5000; IHC: 1: 20-1: 200; IF: 1: 50-1: 200
• Immunogen: Recombinant Human Signal transducer and activator of transcription 5B protein (1-787AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Conjugation: Non-conjugated
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

IHC (Immunohistchemistry)

(Immunohistochemistry of paraffin-embedded human testis tissue using AAA26968 at dilution of 1:100)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human lung cancer using AAA26968 at dilution of 1:100)

IP (Immunoprecipitation)

(Immunoprecipitating STAT5B in K562 whole cell lysateLane 1: Rabbit monoclonal IgG (1ug)instead of AAA26968 in K562 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA26968 (8ug)+ K562 whole cell lysate (500ug)Lane 3: K562 whole cell lysate (10ug))

IHC (Immunohistochemistry)

(Immunofluorescent analysis of HepG2 cells using AAA26968 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human spleen tissue using AAA26968 at dilution of 1:100)

WB (Western Blot)

(Western BlotPositive WB detected in: Jurkat whole cell lysate,K562 whole cell lysate,Rat liver tissue,Mouse liver tiaaue,Mouse lung tissue,Mouse kidney tissueAll lanes: STAT5B antibody at 2.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 90 KDaObserved band size: 90 KDa)

Related Product Information for anti-STAT5B antibody

Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription.

NCBI and Uniprot Product Information

• NCBI GI #: 21618344
• NCBI GeneID: 6777
• NCBI Accession #: NP_036580.2
• NCBI GenBank Nucleotide #: NM_012448.3
• UniProt Accession #: P51692; Q8WWS8
• Molecular Weight: 89,866 Da
• NCBI Official Full Name: signal transducer and activator of transcription 5B
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 5B
• NCBI Official Symbol: STAT5B
• NCBI Official Synonym Symbols: STAT5
• NCBI Protein Information: signal transducer and activator of transcription 5B
• UniProt Protein Name: Signal transducer and activator of transcription 5B
• UniProt Gene Name: STAT5B
• UniProt Entry Name: STA5B_HUMAN