Human C type lectin domain family 4 member A (CLEC4A) ELISA Kit | CLEC4A elisa kit
Human C type lectin domain family 4 member A (CLEC4A) ELISA Kit
Gene Names
CLEC4A; DCIR; LLIR; DDB27; CLECSF6; HDCGC13P
Reactivity
Human
Synonyms
C type lectin domain family 4 member A (CLEC4A); N/A; Human C type lectin domain family 4 member A (CLEC4A) ELISA Kit; CLEC4A elisa kit
Reactivity
Human
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for CLEC4A elisa kit
Intended Uses: This CLEC4A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CLEC4A. This ELISA kit for research use only!
Principle of the Assay: CLEC4A ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CLEC4A antibody and an CLEC4A-HRP conjugate. The assay sample and buffer are incubated together with CLEC4A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLEC4A concentration since CLEC4A from samples and CLEC4A-HRP conjugate compete for the anti-CLEC4A antibody binding site. Since the number of sites is limited, as more sites are occupied by CLEC4A from the sample, fewer sites are left to bind CLEC4A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLEC4A concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CLEC4A ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CLEC4A antibody and an CLEC4A-HRP conjugate. The assay sample and buffer are incubated together with CLEC4A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLEC4A concentration since CLEC4A from samples and CLEC4A-HRP conjugate compete for the anti-CLEC4A antibody binding site. Since the number of sites is limited, as more sites are occupied by CLEC4A from the sample, fewer sites are left to bind CLEC4A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLEC4A concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CLEC4A elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
19,280 Da
NCBI Official Full Name
C-type lectin domain family 4 member A
NCBI Official Synonym Full Names
C-type lectin domain family 4, member A
NCBI Official Symbol
CLEC4A
NCBI Official Synonym Symbols
DCIR; LLIR; DDB27; CLECSF6; HDCGC13P
NCBI Protein Information
C-type lectin domain family 4 member A; C-type lectin DDB27; lectin-like immunoreceptor; dendritic cell immunoreceptor; C-type lectin superfamily member 6; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 6
UniProt Protein Name
C-type lectin domain family 4 member A
UniProt Gene Name
CLEC4A
UniProt Synonym Gene Names
CLECSF6; DCIR; LLIR; HDCGC13P
UniProt Entry Name
CLC4A_HUMAN
