Standard Curve (Sample)
Human Cell division cycle protein 20 homolog (CDC20) ELISA Kit | CDC20 elisa kit
Human Cell division cycle protein 20 homolog (CDC20) ELISA Kit
Gene Names
CDC20; CDC20A; p55CDC; bA276H19.3
Reactivity
Human
Synonyms
Cell division cycle protein 20 homolog (CDC20); Human Cell division cycle protein 20 homolog (CDC20) ELISA Kit; CDC20 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of CDC20. No significant cross-reactivity or interference between CDC20 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CDC20 and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CDC20 elisa kit
Principle of the Assay: CDC20 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CDC20 antibody and an CDC20-HRP conjugate. The assay sample and buffer are incubated together with CDC20-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDC20 concentration since CDC20 from samples and CDC20-HRP conjugate compete for the anti-CDC20 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDC20 from the sample, fewer sites are left to bind CDC20-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDC20 concentration in each sample is interpolated from this standard curve.
Intended Uses: This CDC20 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CDC20. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This CDC20 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CDC20. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Product Categories/Family for CDC20 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,723 Da
NCBI Official Full Name
cell division cycle protein 20 homolog
NCBI Official Synonym Full Names
cell division cycle 20
NCBI Official Symbol
CDC20
NCBI Official Synonym Symbols
CDC20A; p55CDC; bA276H19.3
NCBI Protein Information
cell division cycle protein 20 homolog; cell division cycle 20 homolog; CDC20 cell division cycle 20 homolog
UniProt Protein Name
Cell division cycle protein 20 homolog
UniProt Gene Name
CDC20
UniProt Entry Name
CDC20_HUMAN
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Product Notes
The Human CDC20 cdc20 (Catalog #AAA27181) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA27181 ELISA Kit recognizes Human CDC20. It is sometimes possible for the material contained within the vial of "Cell division cycle protein 20 homolog (CDC20), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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