Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Prl3b1. No significant cross-reactivity or interference between Prl3b1 and analogues was observed.
Sequence Length
222
Assay Type
Competitive
Samples
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Intra-assay Precision
CV<8%
Inter-assay Precision
CV<10%
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Related Product Information for Prl3b1 elisa kit
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
25,159 Da
NCBI Official Full Name
Prolactin-3B1
NCBI Official Synonym Full Names
prolactin family 3, subfamily b, member 1
NCBI Official Symbol
Prl3b1
NCBI Official Synonym Symbols
PL; Pl2; Csh2; Ghd8; Pl-2; mplII; mPL-II
NCBI Protein Information
prolactin-3B1
UniProt Protein Name
Prolactin-3B1
UniProt Gene Name
Prl3b1
UniProt Synonym Gene Names
Csh2; Pl-2; Pl2; PL-II