Standard Curve (Sample)
Human HSD17B1 (Estradiol 17-beta-dehydrogenase 1) ELISA Kit | HSD17B1 elisa kit
Human HSD17B1 (Estradiol 17-beta-dehydrogenase 1) ELISA Kit
Gene Names
HSD17B1; E2DH; HSD17; EDHB17; EDH17B2; SDR28C1; 17-beta-HSD; 20-alpha-HSD
Reactivity
Human
Synonyms
HSD17B1 (Estradiol 17-beta-dehydrogenase 1); Human HSD17B1 (Estradiol 17-beta-dehydrogenase 1) ELISA Kit; HSD17B1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of HS. No significant cross-reactivity or interference between HS and analogues is observed.
Sequence Length
328
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HS were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HS were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for HSD17B1 elisa kit
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with HS. During the reaction, HS in the sample or standard competes with a fixed amount of HS on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to HS. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HS in the samples is then determined by comparing the O D of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
34,950 Da
NCBI Official Full Name
HSD17B1, partial
NCBI Official Synonym Full Names
hydroxysteroid 17-beta dehydrogenase 1
NCBI Official Symbol
HSD17B1
NCBI Official Synonym Symbols
E2DH; HSD17; EDHB17; EDH17B2; SDR28C1; 17-beta-HSD; 20-alpha-HSD
NCBI Protein Information
estradiol 17-beta-dehydrogenase 1
UniProt Protein Name
Estradiol 17-beta-dehydrogenase 1
UniProt Gene Name
HSD17B1
UniProt Synonym Gene Names
E17KSR; EDH17B1; EDH17B2; EDHB17; SDR28C1; 17-beta-HSD 1; 20-alpha-HSD
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Product Notes
The Human HSD17B1 hsd17b1 (Catalog #AAA27593) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA27593 ELISA Kit recognizes Human HSD17B1. It is sometimes possible for the material contained within the vial of "HSD17B1 (Estradiol 17-beta-dehydrogenase 1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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